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Acta cir. bras ; 32(3): 203-210, Mar. 2017. tab
Article Dans Anglais | LILACS | ID: biblio-837689

Résumé

Abstract Purpose: To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats. Methods: In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, five minutes before reperfusion. Comparison groups were isoflurane and propofol sham groups and isoflurane and propofol ischemia-reperfusion-induced renal injury. Renal tubular cell viability was quantitatively assessed by flow cytometry after cell culture and classified as early apoptosis, necrotic cells, and intact cells. Results: Early apoptosis was significantly higher in isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury when compared to both cyclosporine A treated and sham groups. Necrosis percentage was significantly higher in propofol-anesthetized animals subjected to renal ischemia-reperfusion injury. The percentage of intact cells was lower in both, isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury. Conclusion: In a model of ischemia-reperfusion-induced renal injury, cyclosporine A, 5 m.kg-1, administered five minutes before renal reperfusion in rats with acute-induced hyperglycemia under either isoflurano or propofol anesthesia, attenuated early apoptosis and preserved viability in renal tubular cells, regardless of the anesthetic used.


Sujets)
Animaux , Mâle , Lésion d'ischémie-reperfusion/prévention et contrôle , Ciclosporine/pharmacologie , Apoptose/effets des médicaments et des substances chimiques , Agents protecteurs/pharmacologie , Hyperglycémie/physiopathologie , Rein/effets des médicaments et des substances chimiques , Prémédication , Facteurs temps , Lésion d'ischémie-reperfusion/complications , Répartition aléatoire , Propofol/pharmacologie , Survie cellulaire/effets des médicaments et des substances chimiques , Reproductibilité des résultats , Résultat thérapeutique , Rat Wistar , Anesthésiques intraveineux/pharmacologie , Anesthésiques par inhalation/pharmacologie , Cytométrie en flux , Ischémie/prévention et contrôle , Isoflurane/pharmacologie , Rein/vascularisation , Rein/anatomopathologie , Nécrose/prévention et contrôle
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