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1.
Braz. j. med. biol. res ; 32(5): 639-43, May 1999.
Article Dans Anglais | LILACS | ID: lil-233482

Résumé

The collagen structure of isolated and in situ liver granuloma from Swiss Webster mice infected with Schistosoma mansoni was sequentially and three-dimensionally analyzed during different times of infection (early acute, acute, transitional acute-chronic, and chronic phases) by laser scanning confocal microscopy and electron scanning variable vacuum microscopy. The initial granuloma structure is characterized by vascular collagen residues and by anchorage points (or fiber radiation centers), from where collagenous fibers are angularly shed and self-assembled. During the exudative-productive stage, the self-assembly of these fibers minimizes energy and mass through continuous tension and focal compression. The curvature or angles between collagen fibers probably depends on the fibroblastic or myofibroblastic organization of stress fibers. Gradually, the loose unstable lattice of the exudative-productive stage transforms into a highly packed and stable architecture as a result of progressive compactness. The three-dimensional architecture of granulomas provides increased tissue integrity, efficient distribution of soluble compounds and a haptotactic background to the cells


Sujets)
Animaux , Souris , Collagène/analyse , Granulome/anatomopathologie , Maladies du foie/anatomopathologie , Schistosomiase à Schistosoma mansoni/anatomopathologie , Collagène/ultrastructure , Matrice extracellulaire/composition chimique , Matrice extracellulaire/ultrastructure , Fibroblastes , Microscopie confocale
2.
Braz. j. med. biol. res ; 29(1): 19-24, Jan. 1996. ilus
Article Dans Anglais | LILACS | ID: lil-161648

Résumé

Pleural and peritoneal milky spots (MS) are small morphofunctional structures representing subsidiary foci of coelom-associated lymphomyeloid tissue (CALT). In this paper we studied the cellular composition of CALT in normal and Schistosoma mansoni-infected mice. In the healthy mouse, CALT is mainly composed of IgM (+) B cells and presents lower numbers of CD23 and CD45R (B220) B2 lymphocytes. When activated by the infection, it may show pronounced lymphocytosis, plasmocytogenesis (IgM >IgG>IgA>IgG2a>IgG1) and myelomonocytosis. The lymphocytes were mainly of the B1 type (double positive CD5/IgM), with smaller number of T cells (TCR alpha beta (+), TCR gamma delta (+), CD3 (+) and CD5 (+)) and conventional B2 cells (B220 (+), CD23 (+)). The myeloid compartment was composed of immature and mature cells of monocyte/macrophage, eosinophil, neutrophil and megakaryocytic lineages, especially in the omental milky spots. CALT is also a favorable microenvironment for LFA-1 (+) mast cells. Thus, CALT appears to be a mixed lymphoid organ, with secondary and/or primary lymphoid organ functions, being an important site of B1 cell generation, plasma cell maturation and extramedullar hematopoiesis. CALT operates as an interface between blood and lymphatic circulation and coelomic cavities, because locally or externally produced cells have easy and ready access to the pleural and peritoneal cavities. Furthermore, MS cells can escape into blood and lymphatic vessels, providing lymphocytes to other lymphoid organs and to the mucosa-associated lymphoid tissue.


Sujets)
Souris , Animaux , Lymphocytes/anatomopathologie , Tissu lymphoïde/anatomopathologie , Cavité péritonéale/anatomopathologie , Schistosomiase à Schistosoma mansoni/anatomopathologie , Plèvre/anatomopathologie
3.
Mem. Inst. Oswaldo Cruz ; 90(2): 311-318, Mar.-Apr. 1995.
Article Dans Anglais | LILACS | ID: lil-319892

Résumé

Twenty Calomys callosus, Rengger, 1830 (Rodentia-Cricetidae) were studied in the early stage of the acute schistosomal mansoni infection (42nd day). The same number of Swiss Webster mice were used as a comparative standard. Liver and intestinal sections, fixed in formalin-Millonig and embedded in paraffin, were stained with hematoxilin and eosin, PAS-Alcian Blue, pH = 1.0 and 2.5, Lennert's Giemsa, Picrosirius plus polarization microscopy, Periodic acid methanamine silver, Gomori's silver reticulin and resorcin-fuchsin. Immunohistological study (indirect immunofluorescence and peroxidase labeled extravidin-biotin methods) was done with antibodies specific to pro-collagen III, fibronectin, elastin, condroitin-sulfate, tenascin, alpha smooth muscle actin, vimentin and desmin. The hepatic granulomas were small, reaching only 27 of the volume of the hepatic Swiss Webster granuloma. They were composed mainly by large immature macrophages, often filled by schistosomal pigment, characterizing an exsudative-macrophage granuloma type. The granulomas were situated in the parenchyma and in the portal space. They were often intravascular, poor of extracellular matrix components, except fibronectin and presented, sometimes alpha smooth muscle actin and vimentin positive cells. The C. callosus intestinal granulomas were similar to Swiss Webster, showing predominance of macrophages. Therefore, the C. callosus acquire very well the Schistosoma mansoni infection, without developing strong hepatic acute granulomatous reaction, suggesting lack of histopathological signs of hypersensitivity.


Sujets)
Animaux , Souris , Arvicolinae , Maladies des rongeurs/parasitologie , Foie/anatomopathologie , Intestins , Schistosomiase à Schistosoma mansoni , Maladie aigüe , Maladies des rongeurs/anatomopathologie , Parasitoses intestinales/anatomopathologie , Fibrose , Granulome , Parasitoses hépatiques/anatomopathologie , Protéines de la matrice extracellulaire , Rodentia , Schistosomiase à Schistosoma mansoni
4.
Mem. Inst. Oswaldo Cruz ; 90(2): 169-177, Mar.-Apr. 1995.
Article Dans Anglais | LILACS | ID: lil-319904

Résumé

During Schistosoma mansoni infection, there is morphological evidence of involvement of various hematopoietic growth factors, which cause eosinophil, neutrophil, megakaryocytic and erythroid extramedullary foci in the liver, lymph nodes and omental and mesenteric milky spots. While the eosinophil metaplasia in the periphery of hepatic granulomas roughly reproduced the intensity of the medullary eosinopoiesis, the neutrophil metaplasia, on the contrary, was more intense during the period of neutrophil depression in the bone marrow. This fact suggests that extramedullary hematopoietic foci are locally regulated, and amplify and/or compensate the systemic hematopoietic response during the infection.


Sujets)
Animaux , Femelle , Humains , Mâle , Souris , Hématopoïèse extramédullaire , Schistosomiase à Schistosoma mansoni , Foie/anatomopathologie , Granulome , Parasitoses hépatiques/anatomopathologie , Moelle osseuse/anatomopathologie , Métaplasie , Myélofibrose primitive , Facteurs temps
5.
Mem. Inst. Oswaldo Cruz ; 87(supl.5): 111-6, 1992.
Article Dans Anglais | LILACS | ID: lil-128430

Résumé

Milky spots (MS), considered by the authors as a Coelomatic Lympho-myelopoietic Organ (CLMO), present a strong reactivity during experimental schistosomal mansoni infection, characterized by an increase of lymphocytes, macrophages, plasmocytes, mast cells, neutrophils and expression of eosinophil metaplasia. Intraperitoneal injection of purified Schistosoma mansoni (Sm) eggs provoked a rise in the number and size of MS, which developed the sessile marginal and pedunculated types. The authors conclude that egg antigens are, at least partially, responsible for MS reactivity during Sm infection


Sujets)
Granulocytes éosinophiles , Tissu lymphoïde/immunologie , Péritoine , Schistosomiase à Schistosoma mansoni/immunologie
8.
Braz. j. med. biol. res ; 23(10): 989-94, 1990. ilus
Article Dans Anglais | LILACS | ID: lil-91638

Résumé

Eosinophilia in murine schistosomiasis is very intense and extensive, involving distinct compartments such as bone marrow, blood, peritoneal cavity and tissues. Comparison of the shapes of eosinophil concentration or distribution curves showed a synchronization of the tendencies around 50% between blood and bone marrow, 33 to 64% between bloode and peritoneal cavity, and 33 to 43% between peritoneal cavity and bone marrow. The hepatic eosinophil granulocytopoiesis or metaplasia follows the same pattern as observed in bone marrow. Schistosoma infection can be divided into three distinct phases based on the eosinophilic response: 1) non- or low-productive phase (before 35-40 days of infection), 2) acute productive phase (from 35-40 to 70-90 days), and 3) chronic productive phase (after 70-90 days of infection)


Sujets)
Animaux , Granulocytes éosinophiles/physiopathologie , Éosinophilie/étiologie , Moelle osseuse/physiopathologie , Cavité péritonéale/physiopathologie , Schistosomiase à Schistosoma mansoni/sang , Numération des leucocytes
9.
Braz. j. med. biol. res ; 22(9): 1105-9, 1989. tab, ilus
Article Dans Anglais | LILACS | ID: lil-83185

Résumé

Pancreatic involvement during murine schistosomiasis is frequent (30 to 80%), heterogeneous, usually mild, but can occasionally be severe, characterized by granulomatous pancreatitis. After infection, pancreatic granulomas appear from day 50 on, with th most severe pancreatitis being demonstrable between days 90 and 100. Mice thus appear to be a useful model for study of the pathogenesis of Schistosoma mansoni-induced pancreatitis


Sujets)
Souris , Humains , Mâle , Femelle , Pancréatite/étiologie , Schistosomiase à Schistosoma mansoni/complications , Pancréas/vascularisation , Pancréatite/physiopathologie , Schistosomiase à Schistosoma mansoni/physiopathologie , Veines/parasitologie
10.
Braz. j. med. biol. res ; 21(5): 999-1003, 1988. ilus
Article Dans Anglais | LILACS | ID: lil-63597

Résumé

The endothelial cells participate in the morphological events occuring during murine schistosomiasis, taking part in the development of hepatic periovular granuloma. The cells also show an increase in the expression of Factor WIII - related antigen in the portal vessels and hepatic sinusoids during the infection. Endothelial cells are suggested play an important role in the pathogenesis of the disease and in the balance of the coagulant - anticoagulant mechanisms which favor the intravascular survial of the parasites


Sujets)
Rats , Animaux , Endothélium vasculaire/anatomopathologie , Schistosomiase à Schistosoma mansoni/anatomopathologie , Facteur VIII/analyse , Granulome/étiologie , Parasitoses hépatiques/étiologie
11.
Mem. Inst. Oswaldo Cruz ; 82(supl.4): 67-76, 1987. graf, ilus
Article Dans Anglais | LILACS | ID: lil-623666

Résumé

During the schistosomiasis infection there is a [quot ]dance of the cells[quot ], varying from site to site and related to the time of infection. 1 - Eosinophil levels exhibit a bimodal pattern, with the first peak related to the egg deposition and maturation and increased Kupfferian hyperplasia; the second peak precedes the death of some adult worms; 2 - The peritoneal eosinophilic levels are inversely proportional to the blood eosinophilic levels; 3 - Eosinopoiesis in the bone marrow begins at day 40, reaching the highest levels at day 50 and coincides with hepatic eosinophilic and neutrophilic metaplasia; 4 - Peritoneal mast cell levels present a bimodal pattern similar to the blood eosinophils, and inverse to the peritoneal eosinophils. They also show a cyclic behaviour within the hepatic and intestinal granulomas. Integral analysis of the events related to the eosinophils in the blood, bone marrow, peritoneal cavity and hepatic and intestinal granulomas allows the detection of two important eosinophilic phases: the first is due to mobilization and redistribution of the marginal pool and the second originates from eosinophilic production in the bone marrow and liver. The productive phase is characterized by an increase in the number of eosinophils and monocyte/macrophages, and a decrease in neutrophils and stabilization of megakariocytes and erithroid lineages.


Sujets)
Animaux , Femelle , Schistosomiase à Schistosoma mansoni/complications , Schistosomiase à Schistosoma mansoni/transmission , Éosinophilie/étiologie , Numération cellulaire , Division cellulaire , Éosinophilie/complications , Éosinophilie/physiopathologie
12.
Mem. Inst. Oswaldo Cruz ; 82(supl.4): 257-267, 1987. ilus, graf, tab
Article Dans Anglais | LILACS | ID: lil-623704

Résumé

Modification of the immune response to schistosomal infection in children or offspring born to mother R infected with Schistosoma mansoni has been demonstrated in human and in experimental schistosomiasis. One of the hypothesis to explain this fact could be the transfer of circulating antigens and antibodies from mother to foetus through the placenta or from mother to child by milk. The results of this spontaneous transference are controversial in the literature. In an attempt to investigate these questions, we studied one hundred and twenty offspring (Swiss mice), sixty born to infected-mothers (group A) and sixty born to non-infected mothers (group B). These were percutaneously infected with 50 cercariae/mouse, and divided in six sub-groups (20 mice/sub-group), according to the following schedule: after birth (sub-groups A.I and B.I), 10 days old (sub-groups A.II and B.II) and 21 days old (sub-groups A.III and B.III). After the exposure period, the young mice returned to their own mothers for nursing. Six weeks later, the mice were killed. We obtained the following results: 1) There is transference of antibody to cercariae (CAP), adult worms (SWAP) and egg antigens (SEA) from the infected mothers to the offspring, probably through placenta and milk; 2) Offspring born to infected mothers exhibit much less coagulative hepatic necrosis and show a lower number of eggs in the small intestine and a less intense and predominant exsudative stage of the hepatic granulomas when compared with the exsudative-productive stage of the control groups. The findings suggest that congenital and nursing factors can interfere on the development of the schistosomiasis infection, causing an hyporesponse to the eggs.


Sujets)
Animaux , Cochons d'Inde , Souris , Complications infectieuses de la grossesse/physiopathologie , Schistosomiase à Schistosoma mansoni/congénital , Schistosomiase à Schistosoma mansoni/physiopathologie , Anticorps antihelminthe/immunologie , Ovule/immunologie , Complications infectieuses de la grossesse , Échange foetomaternel
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