Detection of pathogenic leptospiral DNA in urine by polymerase chain reaction.

Diagnosis of leptospirosis is currently based on serological tests detecting antibodies against the spirochete. The standard method is the microscopic agglutination test (MAT), which is serovar-specific, requires a period of antibody development, and increases the risk of exposure to viable organisms. Therefore, the present study aimed to develop a rapid, sensitive, specific, and safe method based on the PCR technique to detect pathogenic Leptospira in urine samples. Nested PCR using two sets of primers, external and internal primers, were shown to specifically amplify 16S rRNA target of patho-genic Leptospira. No amplification was observed when DNA from non-pathogenic Leptospira and other non-Leptospira bacteria were used as DNA templates. The method was able to detect as few as 10 leptospires in urine. Therefore, nested PCR approach may be a useful tool for prompt and definitive diagnosis of leptospirosis.

Evaluation of inhouse rapid urease test for detection of Helicobacter pylori from gastric biopsy specimens.

Inhouse rapid urease test for detecting Helicobacter pylori was evaluated. Biopsy specimens were taken for inhouse urease test, commercial rapid urease CLO test, culture, gram stain and histology from the antrium or duodenum of patients who had peptic ulcer. The culture and/or histologic examination and CLO test were used as the gold standard. One hundred and twelve specimens were evaluated. The sensitivity and specificity of the inhouse urease test was 100 per cent and 90 per cent respectively. The inhouse urease test was suitable for detecting Helicobacter pylori from gastric antral biopsy specimens. The medium can be kept in a refrigerator for up to 6 months.