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AIM To study the programmed death effect of trametenolic acid B (TAB) against human gastric cancer HGC-27 cells.METHODS The proliferation of cells was examined by MTT assay;cell apoptosis rate and cell cycle were detected by flow cytometry;cell autophagy was observed by acridine orange staining and transmission electron microscope;expressions of autophagy-related proteins were detected by Western blot.RESULTS 10,20,40 μmol/L Trametenolic acid B had good growth-inhibitory effects on HGC-27 cells,blocked in G0/G1 phase,in a dose-dependent and time-dependent manner,but had no obvious effect on cell apoptosis.After treatment with 20 μmol/L trametenolic acid B for 24 h,a large number of autophagy vacuoles and autophagy bodies were observed under transmission electron microscope,and the proportion of cell autophagy was significantly increased with higher dose of TAB;TAB promoted the transformation of type Ⅰ microtubule-associated protein 1 light chain 3 (LC3 Ⅰ) to type Ⅱ microtubule-associated protein 1 light chain 3 (LC3 Ⅱ),up-regulated the expressions of myosin-like B-cell lymphoma-2 (Bcl-2) interacting protein (Beclin-1) and cyclin-dependent kinase inhibitor 1A (p21),and inhibited the CCND1 gene protein (CyclinD1) expression.CONCLUSION Trametenolic acid B can induce the autophagic death of HGC-27 cells,but it has no significant effect on cell apoptosis.
Résumé
Objective To evaluate the preoperative sleep quality of inpatients and analyze its influencing factors.Methods A self-designed questionnaire,Pittsburgh sleep quality index(PSQI)and self-rating anxiety scale(SAS)were used to investigate the sleep quality and anxiety of 114 inpatients before operation.The two groups were compared in terms of sleep quality and anxiety.The factors influencing the sleep quality were analyzed.Results Of the 114 preoperative inpatients,72.81%(83 cases)had poor sleep quality.The PSQI scores of preoperative inpatients were significantly higher than that in the healthy individuals,but significantly lower than that in the patients with insomnia(P<0.01).The main factors affecting the quality of sleep were fear of surgery,pain and discomfort,feeling strange with the ward settings,and worry about prognosis,totally causing 89.47%of the sleep disorders. Inpatients with poor sleep quality,37.3%was found suffering from anxiety.The rate was significantly higher than that of patients with good sleep quality(P<0.01).Conclusions The incidence of poor sleep quality is relatively high among the preoperative inpatients, mainly caused by fear of surgery,pain and discomfort.In order to improve the sleep quality,it is necessary to perform psychological and comfort intervention according to the sleep status of the patients.