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1.
China Journal of Chinese Materia Medica ; (24): 465-467, 2006.
Article Dans Chinois | WPRIM | ID: wpr-356793

Résumé

<p><b>OBJECTIVE</b>To investigate the effects of processing adjuvants-different types of processing vinegar on effective constituents in rhizoma of Corydalis yanhusuo, and evaluate the quality of different types of vinegar in China.</p><p><b>METHOD</b>The HPLC method was adopted to determine the extraction solubility of dl-tetrahydropalmation and total alkaloids in rhizoma of Corydalis yanhusuo processed by vinegar. The sample extracts were separated on kromasil ODS column with mobile phase of methanol-1% phosphoric acid solution(65:35) and detection wavelength was 280 nm.</p><p><b>RESULT</b>There was a remarkable increase in extraction solubility of dl-tetrahydropalmation and total alkaloids in the rhizoma of Corydalis yanhusuo processed by the vinegar products with high content of total acids or with known trademarks.</p><p><b>CONCLUSION</b>Some types of vinegar with known trademarks had been preliminarily selected for the process of Chinese traditional medicine and they also met the requitrement of processing adjuvants with medicine grade. The results will be benefited to the foundation of standardization of vinegar.</p>


Sujets)
Acide acétique , Classification , Alcaloïdes , Alcaloïdes de type berbérine , Corydalis , Chimie , Plantes médicinales , Chimie , Reproductibilité des résultats , Rhizome , Chimie , Technologie pharmaceutique , Méthodes
2.
Acta Physiologica Sinica ; (6): 243-247, 2004.
Article Dans Anglais | WPRIM | ID: wpr-352786

Résumé

The purpose of this study was to examine the effects of low-dose capsaicin (CAP) on L-type calcium current (I(Ca-L) ) in guinea pig ventricular myocytes and the underlying mechanism. I(Ca-L) was examined in isolated single guinea pig ventricular myocytes by using whole-cell patch clamp technique. CAP (1-25 nmol/L) increased the voltage-dependently activated peak amplitude of I(Ca-L) and downshifted the current-voltage (I-V) curve. CAP (1, 10, 25 nmol/L) increased the peak amplitude of I(Ca-L) from -9.67+/-0.7 pA/pF to -10.21+/-0.8 pA/pF (P>0.05), to -11.37+/-0.8 pA/pF and to -12.84+/-0.9 pA/pF (P<0.05), respectively. CAP 25 nmol/L shifted the steady-state activation curve of I(Ca-L) to the left and changed half activation potential (V(0.5)) from (-20.76+/-2.0) mV to (-26.71+/-3.0) mV (P<0.05), indicating that low-dose CAP may modify the voltage-dependent activation of calcium channel. Low-dose of CAP did not affect the steady-state inactivation curve of I(Ca-L) or half-recovery time of Ca(2+) channel from inactivation. Ruthenium red (RR, 10 micromol/L), a vanilloid receptor (VR1) blocker, antagonized the effects of low-dose CAP. These results suggest that low-dose CAP increases I(Ca-L) mainly by shifting its steady-state activation curve to the left. Such effects may be mediated by VR1.


Sujets)
Animaux , Canaux calciques de type L , Capsaïcine , Pharmacologie , Relation dose-effet des médicaments , Cochons d'Inde , Ventricules cardiaques , Myocytes cardiaques , Métabolisme , Techniques de patch-clamp , Récepteurs des médicaments , Rouge de ruthénium , Pharmacologie
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