Non-surgical treatment of lumbar intervertebral disc protrusion without blood stasis:a serum proteomic analysis / 中国组织工程研究

BACKGROUND: Proteomics is a well studied research method, but its application in the non-surgical treatment of lumbar intervertebral disc protrusion (LIDP) is little reported. OBJECTIVE: To screen the differentially expressed proteins in patients with LIDP but without blood stasis before and after non-surgical treatment by proteomics. METHODS: Sixty patients with LIDP but without blood stasis were selected, and treated with non-surgical treatment for 4 weeks. The differentially expressed proteins were screened and identified by iTRAQ combined with LC-MS/MS. The bioinformatics analysis of the identified proteins was carried out, and the curative effectiveness was investigated. RESULTS AND CONCLUSION: Compared with those before treatment, the Visual Analogue Scale scores were significantly (P ＜ 0.05), the Japanese Orthopedic Association scores were significantly increased decreased (P ＜ 0.05), and the excellent and good rate reached 95.0% post-treatment. A total of 300 differentially expressed proteins were screened and 25 significantly expressed proteins were identified (P ＜0.05). Bioinformatics analysis revealed that nine of the significantly expressed proteins were enriched to 15 KEGG signaling pathways. These results suggest that the use of Western medicine non-surgical treatment for the LIDP without blood stasis can achieve satisfactory results. Besides, complement C1qA, cDNA protein (FLJ60724), complement C4B frameshift mutation, cDNA protein (FLJ53025), mannose binding protein C, apolipoprotein B, hemoglobin α-1 globin chain variant, hemoglobin β subunit and cDNA protein (FLJ76254) may be the potential serum markers of the non-surgical treatment for the LIDP without non-blood stasis.

Research advance in the effects of androgen and its receptor on the development of obesity, obesity-related diseases and disorders of glucose and lipid metabolism / 生理学报

Recently, there has been a growing interest in the roles of androgen in the development of obesity and obesity-related diseases such as diabetes, metabolic syndrome, atherosclerosis (AS), hypertension and cardiovascular diseases, as well as in the disorders of glucose and lipid metabolism. Androgen exerts its biological role mainly through binding to the androgen receptor (AR), one of the nuclear receptors widely expressed in multiple tissues such as the skeletal muscle, liver, adipose tissue and brain. Low testosterone and AR deficiency result in obesity, obesity-related diseases and metabolic disorders. Androgen regulates almost all the intracellular transduction pathways related to glucose and lipid metabolism, including key metabolic enzymes/proteins, nuclear transcription factors (PPARγ, LXRα and FoxO1), inflammation, leptin sensitivity of hypothalamus, proliferation and differentiation of adipocyte, mitochondrial function and vascular endothelial function. In addition, females with high androgen are more likely to develop obesity as well as glucose and lipid metabolism disorders, while the underlying mechanisms remain unclear. This review focuses on the role and underlying mechanisms of androgen and AR in the development of obesity and obesity-related diseases as well as glucose and lipid metabolism disturbance in men.

Herpes zoster treated with meridian-collateral electric information therapy combined with pricking and cupping / 中国针灸

<p><b>OBJECTIVE</b>To explore the best acupoints for the treatment of herpes zoster.</p><p><b>METHODS</b>Two hundred cases were randomized into an observation group and a control group, 100 cases in each one. In observation group, meridian-collateral electric information diagnosis and treatment instrument was used to detect meridian and collateral so as to find out the relevant "sick meridian open" for electric stimulation, bloodletting and cupping. In control group, Acyclovir was administered orally.</p><p><b>RESULTS</b>In observation group, it had been dectcted that "sick meridian open" were mostly localized in Ashi point (Extra), Zhangmen (LR 13), Daimai (GB 26), Qimen (LR 14), Dabao (SP 15), etc. The totally effective rate in observation group was 100.0% (100/100) that was superior to 60.0% (60/100) in control group (P < 0.000 1). Additionally, the time for pain relief, blister relief and scarring in observation group was shorter obviously than that in control group (P < 0.000 1). There was no case of post-herpetic neuralgia in observation group, but the incidence of it in control group was 26.0% (26/100).</p><p><b>CONCLUSION</b>Meridian-collateral diagnosis and treatment instrument detects "sick meridian open" for electric stimulation and bloodletting and cupping in the treatment of herpes zoster, which can effectively relieve pain and prevent from post-herpetic neuralgia promptly.</p>

Genetic imprinting and embryonic development / 浙江大学学报·医学版

Erasure, establishment and maintenance of genetic imprinting are indispensable for normal embryonic development. All these processes depend on accurate expression and intimate cooperation of kinds of DNA methyltransferases. Many genetic syndromes and embryo developmental anomalies are caused by abnormality of genetic imprinting. Genetic imprinting is important for the nucleus totipotential of primordial germ cell, maturation of gamete,growth and development of embryo, structure and function of placenta as well as postnatal growth and development of individuals.