Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 1 de 1
Filtre
Ajouter des filtres








Gamme d'année
1.
Biol. Res ; 45(3): 297-305, 2012. ilus
Article Dans Anglais | LILACS | ID: lil-659287

Résumé

Prostate cancer (PCa) is the most frequently diagnosed malignancy in men worldwide. Chemotherapy response is very poor and resistance to hormone-based treatments is frequent in advances stages. Recently, tumor-initiating cells or cancer stem cells (CSCs) have been identified in several cancers, including PCa. These cells are thought to be responsible for therapy resistance, relapse and metastasis. In the present work, enriched populations of CSCs were obtained using a mixed procedure that included differential clone-forming ability, sphere growing induction (prostatospheres) and magnetic-associated cell sorting (MACS). Also, stem marker expression was determined in PCa biopsies of different histological grades and metastasis samples. The signature for stem markers of the isolated CSCs was CD133+/CD44+/ABCG2+/ CD24-. Expression of stem markers (CD133, CD44, and ABCG2) was higher in medium Gleason biopsies than in lower and higher grades, and lymph-node and bone metastasis samples. These results suggest that the CSCs in PCa reach an important number in medium Gleason grades, when the tumor is still confined into the gland. At this stage, the surgical treatment is usually with curative intention. However, an important percentage of patients relapse after treatment. Number and signature of CSCs may be a prognosis factor for PCa recurrence.


Sujets)
Humains , Mâle , Antigènes CD/analyse , Régulation de l'expression des gènes tumoraux , Cellules souches tumorales/anatomopathologie , Tumeurs de la prostate/génétique , Biopsie , Tumeurs osseuses/secondaire , Séparation cellulaire , Immunohistochimie , Métastase lymphatique/anatomopathologie , Grading des tumeurs , Métastase tumorale , Récidive tumorale locale , Pronostic , Tumeurs de la prostate/anatomopathologie , Test clonogénique de cellules souches tumorales , Marqueurs biologiques tumoraux/analyse
SÉLECTION CITATIONS
Détails de la recherche