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1.
Braz. j. med. biol. res ; 45(8): 777-783, Aug. 2012. ilus, tab
Article Dans Anglais | LILACS | ID: lil-643662

Résumé

We examined the capacity of high-intensity intermittent training (HI-IT) to facilitate the delivery of lipids to enzymes responsible for oxidation, a task performed by the carnitine palmitoyl transferase (CPT) system in the rat gastrocnemius muscle. Male adult Wistar rats (160-250 g) were randomly distributed into 3 groups: sedentary (Sed, N = 5), HI-IT (N = 10), and moderate-intensity continuous training (MI-CT, N = 10). The trained groups were exercised for 8 weeks with a 10% (HI-IT) and a 5% (MI-CT) overload. The HI-IT group presented 11.8% decreased weight gain compared to the Sed group. The maximal activities of CPT-I, CPT-II, and citrate synthase were all increased in the HI-IT group compared to the Sed group (P < 0.01), as also was gene expression, measured by RT-PCR, of fatty acid binding protein (FABP; P < 0.01) and lipoprotein lipase (LPL; P < 0.05). Lactate dehydrogenase also presented a higher maximal activity (nmol·min-1·mg protein-1) in HI-IT (around 83%). We suggest that 8 weeks of HI-IT enhance mitochondrial lipid transport capacity thus facilitating the oxidation process in the gastrocnemius muscle. This adaptation may also be associated with the decrease in weight gain observed in the animals and was concomitant to a higher gene expression of both FABP and LPL in HI-IT, suggesting that intermittent exercise is a "time-efficient" strategy inducing metabolic adaptation.


Sujets)
Animaux , Mâle , Rats , Carnitine O-palmitoyltransferase/métabolisme , Muscles squelettiques/enzymologie , Conditionnement physique d'animal/physiologie , Adaptation physiologique/physiologie , Lipoprotein lipase/métabolisme , Muscles squelettiques/métabolisme , Muscles squelettiques/physiologie , Oxydoréduction , Répartition aléatoire , Rat Wistar , Réaction de polymérisation en chaine en temps réel , RT-PCR
2.
Rev. bras. anal. clin ; 31(4): 205-206, 1999. tab
Article Dans Portugais | LILACS | ID: lil-522810

Résumé

Foram analisadas amostras de fezes, material da região ungueal e a água após a lavagem das mãos de 58 crianças e adolescentes, entre 12 a 17 anos de idade, da Instituição Filantrópica de Marília, no período de 01 de agosto a 30 de setembro de 1988. Dos 58 pacientes analisados, 17 (29,3%) não apresentaram nenhum tipo de infecção parasitária; 41 (70,7%) apresentaram-se parasitados por protozoários com a prevalência de 35,1% do tipo Giardia lamblia em 5 (11,8%) e Entamoeba coli em 10 (23,3%); os demais, ou seja, 64,9% apresentaram-se parasitados por helmintos do tipo Ascaris lumbricoides em 10 (23,3), Ancilostomideos em 3 (8,3%) e Hymenolepis nana em 13 (33,3%). Diante dos resultados obtidos, pode-se verificar que o foco das infecções apresentadas, não teve correlação com a região ungueal ou com as mãos contaminadas, uma vez que os cistos, larvas e ovos pesquisados foram encontrados apenas nas fezes analisadas.


Sujets)
Humains , Mâle , Femelle , Enfant , Adolescent , Contamination des aliments/prévention et contrôle , Hygiène/analyse , Parasitoses intestinales
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