Résumé
This study aims to clarify out the anti-inflammatory mechanism of Qingfei Xiaoyan Wan. Chemical constituents of Qingfei Xiaoyan Wan identified by UPLC Q-TOF, were submit to Molinspiration, PharmMapper and KEGG bioinformatics softwares for predicting their absorption parameters, target proteins and related pathways respectively; and the gene chip and real time-PCR were carried out to investigate the expression of inflammatory genes on lung tissue of guinea pigs or human bronchial epithelial cell lines. The predicted results showed that 19 of the 24 absorbable constituents affected at 9 inflammation-related pathways through 11 protein targets; Qingfei Xiaoyan Wan treatment can significantly reduce the infiltration of cytokines through ERK1 gene and 5 inflammatory pathways (Focal adhesion, Fc epsilon RI, Toll-like receptors, NK cell-mediated cytotoxic, and ERK/MAPK). The results of real time-PCR further confirmed that the anti-inflammatory effects of Qingfei Xiaoyan Wan were due to active ingredients such as arctigenin, cholic acid and sinapic acid intervened focal adhesion, Fc epsilon RI signaling and ERK/MAPK pathways. The novel approach of 'drug-target-pathway' will present an effective strategy for the study of traditional Chinese medicines.
Sujets)
Animaux , Femelle , Humains , Mâle , Anti-inflammatoires , Pharmacologie , Asthme , Métabolisme , Anatomopathologie , Lignée cellulaire , Acide cholique , Pharmacologie , Acides coumariques , Pharmacologie , Cytokines , Métabolisme , Association médicamenteuse , Médicaments issus de plantes chinoises , Pharmacologie , Cellules épithéliales , Furanes , Pharmacologie , Cochons d'Inde , Inflammation , Métabolisme , Lignanes , Pharmacologie , Poumon , Anatomopathologie , Système de signalisation des MAP kinases , Répartition aléatoire , Récepteurs aux IgE , Métabolisme , Récepteurs de type Toll , MétabolismeRésumé
OBJECTIVE: To develop a UPLC-Q-TOF-MS method for determination of six components (guanosine, peiminine, platycodin D, platycodin D3, oleanolic acid and ursolic acid) in Zhikechuanbeipipa dropping pills. METHODS: The analysis was performed on a Waters Acquity BEH C18 column (2.1 mm×100 mm, 1.7 μm) with the mixture of acetonitrile-water-formic acid as mobile phase, and the flow rate was 0.4 mL·min-1; The PDA detection wavelength was 190-400 nm; The column temperature was room temperature (30°C). RESULTS: The linear relationship between the concentration and peak areas of the six compounds were all linear (r>0.9996). The average recoveries (n=6) were between 99%-101%. CONCLUSION: This method was simple, accurate and reliable to determine the six contents of Zhikechuanbeipipa dropping pills for the quality control. Copyright 2012 by the Chinese Pharmaceutical Association.