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1.
Acta Pharmaceutica Sinica ; (12): 986-992, 2015.
Article Dans Chinois | WPRIM | ID: wpr-257037

Résumé

The aim of this study is to investigate the anti-inflammatory effect of the adenosine derivative N6-(3-hydroxylaniline) adenosine (WS070117M1) on cigarette smoke plus LPS (lipopolysaccharide)-induced chronic obstructive pulmonary disease (COPD) in mice and its mechanism. COPD model was established by exposing male BALB/c mice to cigarette smoke and challenged with LPS inhalation. Supernatants of bronchoalveolar lavage fluid (BALF) were harvested and IL-1β, IL-6, IL-8 and TGF-β1 levels were measured by ELISA (enzyme-linked immunesorbent assay). The number of total white blood cells and neutrophils in bronchoalveolar lavage fluid was counted separately. Lung tissue was stained with Mayer 's hematoxylin and eosin for histopathologic examination. pAMPKa protein expression and distribution of lung tissue were analyzed by immunohistochemistry method. In vitro, levels of AMPKα phosphorylation in phorbol-12- myristate-13-acetate (PMA) differentiated THP-1 cells was detected by immunohistochemistry, IL-8 level in supernatants of cigarette smoke condensate stimulating PMA differentiated THP-1 cells was measured by ELISA. The results showed that WS070117M1 treatment significantly activated AMPKa in the lung tissue. It also resulted in down regulation of IL-1β, IL-6, IL-8 and TGF-β1 levels in bronchoalveolar lavage fluid and IL-8 level in cigarette smoke condensate stimulating PMA differentiated THP-1 cells. In addition, WS070117M1 could inhibit the recruitment of total white blood cells and neutrophils. These results suggest that WS070117M1 may alleviate the airway inflammation by activating AMPK in the lung tissue.


Sujets)
Animaux , Humains , Mâle , Souris , AMP-Activated Protein Kinases , Métabolisme , Adénosine , Liquide de lavage bronchoalvéolaire , Lignée cellulaire tumorale , Modèles animaux de maladie humaine , Inflammation , Traitement médicamenteux , Interleukine-1 bêta , Métabolisme , Interleukine-6 , Métabolisme , Interleukine-8 , Métabolisme , Numération des leucocytes , Lipopolysaccharides , Souris de lignée BALB C , Granulocytes neutrophiles , Biologie cellulaire , Broncho-pneumopathie chronique obstructive , Traitement médicamenteux , Fumée , Nicotiana , Facteur de croissance transformant bêta-1 , Métabolisme
2.
Acta Pharmaceutica Sinica ; (12): 1089-1096, 2014.
Article Dans Chinois | WPRIM | ID: wpr-299163

Résumé

AMP-activated protein kinase (AMPK) is an important regulator of cellular energy homeostasis. Recent studies demonstrated that AMPK is a novel signaling molecule modulating inflammatory responses and oxidative stress which are involved in inflammatory pulmonary diseases, such as asthma, chronic obstructive pulmonary disease (COPD), pulmonary infectious diseases and pulmonary fibrosis. AMPK attenuates inflammatory lung injury by phosphorylating its downstream targets, such as sirtuin1 (SIRT1), peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha), p53 and forkhead box O3a (FoxO3a). This review summarized the relationship between AMPK and the development of inflammatory pulmonary diseases.


Sujets)
Humains , AMP-Activated Protein Kinases , Métabolisme , Protéine O3 à motif en tête de fourche , Facteurs de transcription Forkhead , Métabolisme , Homéostasie , Inflammation , Maladies pulmonaires , Stress oxydatif , Récepteur PPAR gamma , Métabolisme , Coactivateur 1-alpha du récepteur gamma activé par les proliférateurs de peroxysomes , Phosphorylation , Sirtuine-1 , Métabolisme , Facteurs de transcription , Métabolisme , Protéine p53 suppresseur de tumeur , Métabolisme
3.
Acta Pharmaceutica Sinica ; (12): 1503-1508, 2010.
Article Dans Chinois | WPRIM | ID: wpr-250603

Résumé

The aim of the present study is to investigate the effects of Vam3 which is one of the dihydroxystilbene compounds on expressions of ICAM-1 in the lungs of OVA-induced asthmatic mice and the mechanisms of anti-airway inflammation. Balb/c mice were challenged with OVA inhalation. Lung tissues were stained with Mayer's hematoxylin and eosin for histopathologic examination. The expression of ICAM-1 in the lungs of mice was analyzed by Western blotting and immunohistochemistry method. The NF-kappaB activities were detected by NF-kappaB-luc reporter genetic transient transfection method. The activities of MMP-9 induced by LPS, TNF-alpha and PMA in THP-1 cells were determined by gelatin zymography method. The results showed that Vam3 could inhibit the expression of ICAM-1 in the OVA-induced mouse model. In addition, Vam3 could significantly suppress the activities of NF-kappaB in A549 cells and MMP-9 in THP-1 cells induced by LPS, TNF-alpha and PMA. These results suggested that Vam3 could alleviate the asthmatic inflammation by decreasing ICAM-1 expression in asthmatic mice, down regulating NF-kappaB and MMP-9 activities. Compound Vam3 showed inhibitory effects on inflammatory signal pathways involved in asthma.


Sujets)
Animaux , Humains , Mâle , Souris , Antiasthmatiques , Pharmacologie , Anti-inflammatoires , Pharmacologie , Asthme , Métabolisme , Benzofuranes , Pharmacologie , Lignée cellulaire tumorale , Inflammation , Métabolisme , Molécule-1 d'adhérence intercellulaire , Métabolisme , Leucémie myéloïde , Métabolisme , Anatomopathologie , Poumon , Métabolisme , Anatomopathologie , Tumeurs du poumon , Métabolisme , Anatomopathologie , Matrix metalloproteinase 9 , Métabolisme , Souris de lignée BALB C , Facteur de transcription NF-kappa B , Métabolisme , Ovalbumine , Stilbènes , Pharmacologie
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