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Basic knowledge of forensic science is theoretical and abstract,therefore,confir-matory experiment teaching is often adopted in the experiment teaching. International education has become an important topic in medical colleges and universities. Forensic medicine is a compulsory subject for medical international students. This article discussed the exploration and practice in teach-ing forensic medicine for international students at Wenzhou Medical University,which included faculty constructing and training,encouraging whole English teaching by young teachers,applying heuristic interactive teaching and case teaching,establishing strict attendance and appraisal system and con-ducting comprehensive evaluation on medical teaching quality and teaching management.
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Aim To investigate the effect of ligustrazine on pulmonary arterial pressure,thromboxane A2(TXA2) and prostacyclin(PGI2) in chronic hypoxic hypercapnic rats.Methods Thirty rats were randomly divided into control group(A), hypoxic hypercapnic group(B) and hypoxic hypercapnia+ ligustrazine (lig) group (C) .Results The mPAP in group B was significantly higher than that in group A(P0.05) ; The specific value of WA/TA(vessel wall area/total area) and SMC (the density of medial smooth muscle cells) were significantly higher in group B than that in group A(P0.05).Conclusion Ligustrazine can inhibit pulmonary hypertension and structural remodeling of pulmonary artery in chronic hypoxic hypercapnic rats by inhibiting synthesis of TXA2.
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AIM: To investigate the effect of diltiazem on mean pulmonary arterial pressure(mPAP) and nitric oxide synthase(NOS) in arterioles in chronic hypoxic hypercapnic rats. METHODS: Twenty-four rats were randomly divided into three groups: control group(A),hypoxic hypercapnic group(B), hypoxic hypercapnia+ diltiazem group (C), constitutive endothelial NOS(ceNOS) were observed in arterioles of rats using the technique of immunohistochemistry,ceNOS mRNA were observed by the technique of in situ hybridization . RESULTS: (1)mPAP was significantly higher in rats of B group than that of A and C group( P 0 05),but mCAP was lower in rats of C group than that in B group.(2)Light microscopy showed WA/TA (vessel wall area/total area) was significantly lower in rats of C group than that of B group ( P
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AIM:To study the relationship between apoptosis, proliferation and expression,mutation of related genes in breast cancer. METHODS: Methods of TUNEL, immunohistochemical S-P and PCR-SSCP were used respectively to study apoptotic index (AI), mitotic index(MI), expression of Bcl-2,p53,c-erbB-2,PCNA,Ki67,TopoⅡ and mutation of p53 in 54 cases of breast cancer. RESULTS: AI and MI were 9.40?3 78 and 5 96?2 36, respectively. There was a significant direct correlation between them( r= 0 46 P
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S. D rats were sensitized with PHA and were killed after two weeks. Peritoneal MC were collect-ed and separated using Arabic gum separating solution. Those MC were attacked by adding PHA andfol1owed by incubating at 37 ?C in water bath. After incubating for 5, 1O, l5, 2O, 30min and Ih,eachincubating medium was aspirated. The MC peroxidase was separated by disc electrophoresis and wasstained by ascrobic acid-benzidine stain. The relative peroxidase activity was calculated. Mean while,1O drops of 20 minute incubating medium were aspirated, fixed,embeded,sectioned,stainedand exam-ined under the electronic microscope routinely- 35 different kinds of 10 S. D. rat, s tissues were cut,embeded,sectioned,and stained with the toluidine blue and examined under light microscope for ob-serving the tissue distribution of MC. The results were as follows: (l )The peroxidase released fromthe PHA attacked MC was successfully demonstrated; (2)As comparing with the c0ntr0l group, theincreasing of the relative peroxidase activity in experirnental groups was not obvious at the intervals of5min and 10min incubating, but was obvious as the intervals of 15~3Omin incubating. No significantincreasing of the relative peroxidase activity was found at 60min incubating1 (3)When incubating for2Omin,the degranulation ratio of the experimental groups was 76%,while that of the control groupswas l6% only. There were two kinds of vacuoles in the degranulating MC plasrna, vacuoles withmembrane and without rnembrane. (4 ) The MC in skin, lung, respiratory tract, digestive tract, boneand thymus were prevalent. The order of MC number decreasing was as follows:urinary tract,repro-ductive tract, heart, liver, spleen, kidney. No MC was found in brain, spinal cord, hypophysis andtestls.