Résumé
Objective: To investigate the effect of endocrine gland-derived vascular endothelial growth factor (EG-VEGF) on the malignant behavior of colon cancer LoVo cells. Methods: The LoVo cells treated with different concentrations (50, 100, and 200 μg · L-1) of EG-VEGF were used as EG-VEGF groups, and the colon cancer LoVo cells cultured with solution without EG-VEGF were regarded as control group. The proliferation activity of LoVo cells was determined by MTT method; flow cytometry (FCM) was used to detect the percentages of LoVo cells at different cell cycles in various groups; Wound scratch assay test and Transwell cell migration assay were used to detect the migration rates of LoVo cells and the number of migration cells. Results: The MTT results showed that compared with control group, the proliferation activities of LoVo cells in 50, 100, and 200 μg · L-1 EG-VEGF groups were increased significantly (P<0. 05); with the increasing of concentration of EG-VEGF, the proliferation activity was increased apparently. The FCM results showed that compared with control group, the percentage of colon cancer LoVo cells at G0/Gi phase in 100 μg · L-1 EG-VEGF group was decreased (P<0. 05) and the percentage of colon cancer LoVo cells at S phase was increased (P<0. 05), but the percentage of colon cancer LoVo cells at G2 + M phase did not change significantly. The wound scratch assay results showed that compared with control group, the migration rate of LoVo cells in 100 μg · L-1EG-VEGF group was increased (P< 0. 05). The Transwell assay results showed that compared with control group, the number of migration cells in 100 μg · L-1 EG-VEGF group was increased significantly (P<0. 05). Conclusion: EG-VEGF can obviously promote the proliferation and migration of colon cancer LoVo cells, and EG-VEGF may be associated with the malignant development of colon cancer.