RÉSUMÉ
Magnetic cell sorting technology is a highly specific and rapid cell sorting technology using superparamagnetic nanocomposites for cell sorting, which is widely used in immunology, stem cytology, oncology, clinical medicine and other fields. Magnetic cell sorting technology is divided into positive isolation, negative isolation/untouched cell isolation, depletion, multi-step isolation and automated cell separation systems. In this review, we firstly give a brief introduction to the classification and application of magnetic cell sorting technology, then discuss several new techniques and challenges based on magnetic cell sorting in recent years, such as improving the sorting efficiency by improving the structure of magnetic materials and magnetic field structure. The necessity of biological evaluation of magnetic cell sorting products was emphatically analyzed. Through the biological evaluation, the advantages and disadvantages of magnetic cell sorting products can be understood, and the research and development ability could be improved. Therefore, 10 biological evaluation technical parameters related to magnetic cell sorting products were proposed: yield, purity, sterility, cytotoxicity, cell morphology, viability, light scattering characteristics of cells, fluorescent antibody labeling ability of cells, cell activation and cell proliferation. The 10 biological evaluation technical parameters play an important role in promoting the standardized application of magnetic cell sorting.
RÉSUMÉ
Polycystic ovary syndrome(PCOS)is the most common endocrine and metabolic disorder of childbearing-age women,with clinical symptoms in⁃cluding ovulatory dysfunction and hyperandrogen⁃ism.Diagnosis of PCOS must exclude other causes of ovulation disorders and hyperandrogenism.
RÉSUMÉ
Objective To use ultrasound-guided percutaneous microwave for simultaneous ablation of a nodule in submandibular gland and another in the ipsilateral thyroid gland. Methods A 57-year old man presented non-painful nodules in his right submandibular gland and right thyroid lobe simultaneously, with the sizes being 10 mm×10 mm×9 mm and 13 mm× 11 mm×10 mm separately. Percutaneous microwave ablation and biopsy were conducted for both nodules simultaneously under ultrasound guidance. The short-term effects were documented both clinically and ultrasonographically. Results The ablation therapy was completed uneventfully and took a total of 20 min. The ablation time was 185 seconds for the submandibular nodule and 72 seconds for the thyroid nodule. No intra-operative complications such as bleeding, edema or nerve injury were observed. No symptoms of injuries to the facial nerve, glossal nerve or recurrent laryngeal nerve were observed during a 2-month follow-up. Ultrasound examination revealed that the ablated area was shrank and kept unenhanced on CEUS. Conclusion Microwave ablation therapy is suitable for the conjoint treatment of concomitant nodular lesions in multiple neck organs. The method is economical, efficient, minimally invasive, and is worth of more clinical practices.