Immunization trial by leishmania treated with Mitomycin-c

Leishmania tropica is a protozoan parasite that causes cutaneous disease in the old world. The study aimed to determine cell-mediated immunity by measuring serum IFN-gamma and IL-4 levels, in BALB/C mice immunized with 107 mitomycin-c treated live leishmania promastigotes [inhl], combined with 2microg IL-12, or immunized with 2 microg IL-12 only[controls], or immunized with 107 wild type leishmania promastigotes. We found that inhl induce significantly higher levels of IFN-gamma 360.62 pg/ml than controls 4.36 pg/ml and then WT 41.72 pg/ml. beside of this, inhl induce significantly lower IL-4 levels 3.18 pg/ml than WT 69.82 pg/ml and then controls [lower than 2pg/ml]. Our study revealed that inhl induce cellular mediated immunity comparable with controls and WT

Study for the immune response against some major antigens of leishmania

Leishmaniasis is condidered to be dispersed in Syria, and because of the side effects that caused by drugs and resistant to them, so the prevention is very important to stop dispersing the disease. For that, our study aimed to evaluate the role of cellular immune response [IFN-gamma] and humoral immune response [IgG] that induced by the total extract of L. tropica or the mixed of 63 and 43 KDa antigens which are taken from the same strain. In this study we used fifteen BALB/c mice divided into three groups. The first was immunized by total extract of L. tropica with incomplete frond adjuvant [IFA], and the second group was immunized with the mixed of two proteins. 43, 63 KDa with IFA, and the control group was inoculated with phosphate saline buffer [PBS]. we observed that each total extract and mixed proteins induce Th1 response and possible Th2 response, but this response was significantly stronger with the mixed proteins p=3.6x10-5 for IFN-gamma titer and p=4.8x10-6 for IgG titer which is considered a positive signal to continue studying to reach to an effective and safe vaccine