Pleurodesis using talc

Talc was used for pleurodesis in 30 patients of various pathology [10 with benign effusion due to hepatic failure, 16 for malignant pleural effusion and 4 cases for recurrent spontaneous pneumothorax]. The results are excellent in patients with spontaneous pneumothorax [100%] followed by malignant effusion [75%] and then benign effusion [75%]. Talc proved to be most effective and cheapest agent pleurodesis

Study of pulmonary function tests in insulin dependent diabetes mellitus

Thirty patients complaining from insulin dependent diabetes mellitus were selected for the present study. A set of ventilatory lung function parameters was measured in each patient [N=30] and control subjects [N=10] using an electronic spirometer. The studied group showed a highly significant [p<0.001] reduction in SVC, FEV1, FEV1 / FVC, FEF 25-75% and PEFR when compared with control group. The present study showed insignificant difference in the spirometric parameters when diabetic group with duration of diabetes more than 5 years compared with diabetic group with duration of diabetes 5 years or less. Also, insignificant difference were observed in patients with blood glucose level above 200 mg/dl and those with blood glucose below 200 mg/dl. From the present study we concluded that both restrictive and obstructive ventilatory defect were observed in insulin dependent diabetes mellitus. These ventilatory disorders had no relation to both the duration and glycemic control of diabetes We recommend the periodic evaluation of pulmonary function using spirometer in insulin dependent diabetic patients even though not symptomatized and to remember that the base line is not the same as for healthy subjects

Comparison between solid phase ELISA, dot ELISA and agar gel precipitation test for detection of bursal disease viral antigen in bursal homogenates

Forty-four bursal samples were collected from naturally infected and normal broiler chicks. The samples were homogenized and the homogenates were tested by agar gel precipitation test [AGPT], solid- phase ELISA [by indirect double antibodies s and wich and single antibody ELISA systems] and dot-ELISA for detection of infectious bursal disease virus [IBDv] antigen. The reacted positive samples showed 86.3, 88.6 and 88.6 percentage in AGPT, solid-phase ELISA and dot-ELISA, respectively. The findings suggested that the Dot-ELISA is faster, more economic and easily applicable procedure than the other two techniques. The indirect double antibody s and wich technique is more sensitive than the indirect single antibody ELISA system