use of new markers for the detection of liver damage in experimental rats administered certain heavy metals

Pollution either environmental or industrial becomes one of the most serious problems in this century. In a previous research dealing with the same subject, groups of male healthy workers were selected and recruited from some industrial locations [melting units of different metal factories]. Certain parameters were determined and the results obtained revealed that, neither serum liver enzymes, nor heavy metals level recorded any alterations. Fibronectin and chondroitin sulfate on the other hand, demonstrated significant increase which may be considered as an early marker for liver cirrhosis which may happen later on. The present work was suggested using experimental animals, which received the same heavy metals [Fe, Cu and Pb] for Certain periods aiming to support and confirm the previously observed clinical findings through the examination of liver tissues histopathologically

possible alteration in some lipid metabolic aspects in rats during vitamin C and B6 monotherapy

The effect of vitamins C and B6 monotherapy on some lipid metabolic aspects was studied in normal and alloxan diabetic rats. Long-term administration of vitamin C [30 mg/kg/day] and vitamin B6 [14 mg/kg/ day] induced significant reduction in serum total cholesterol triglycerides and NEFA levels, serum LDL-cholesterol levels decreased significantly after vitamin C treatment while, the change during vitamin B6 therapy is up and down. Serum HDL-cholesterol was markedly increased while, VLDL-cholesterol was mostly nonsignificantly changed following the administration of the two vitamins. Furthermore, vitamin C and B6 supplementation reduced lipid peroxidation in normal and diabetic groups. The results suggested that, the therapeutic doses of vitamins C and B6 long-term therapy increase LDL metabolism and produces hypocholesterolemia in male rats. On the other hand, vitamins C and B6 has a beneficial effect on cardiovascular risk profile

Vitamins prevention of diabetic complication: effect of vitamins C and B6 on glycosylated hemoglobin and sorbitol dehydrogenase

The experiments were undertaken to assess the role of vitamin C [ascorbic acid] and vitamin B6 [pyridoxine] in relation to sorbitol dehydrogenase activity and glycosylated hemoglobin levels in sorbitol pathway linked neural and vascular dysfunction and nonenzymatic glycosylation in rats with alloxan induced diabetes. Vitamins C and B6 monotherapy were given orally to control and diabetic rats. After 30 days and 45 days of treatment, glycosylated hemoglobin levels were decreased significantly [p <0.05 to 0.001]. Sorbitol dehydrogenase activity in normal rats during vitamin C therapy were increased significantly [p <0.08 to 0.001] from 7 to 9 folds after 30 days and 45 days, respectively. In diabetic rats sorbitol dehydrogenase activity elevated about 2.5 folds. Vitamin B6 increased sorbitol dehydrogenase activity about 7 folds in normal and 3 to 2.5 folds after 30 days and 45 days, respectively, in diabetic rats. These observations together with other evidence, suggested the significant relationship between glycosylated hemoglobin, sorbitol dehydrogenase and intake of vitamin C and vitamin B6. The elevated sorbitol dehydrogenase activity associated with the previously reported sorbitol levels. These vitamins supplementation is effective in individuals with insulin-dependent diabetes mellitus [IDDM]. They may be potentially important in controlling glucose-induced nonenzymatic glycosylation of proteins and, therefore may be a preferable drugs for inhibiting glucose induced nonenzymatic glycosylation, neural and vascular dysfunction