Antimicrobial flavonoids from solanum dobium fres

Two flavonoids compounds were isolated from Solanum dobium Fers [Solanaceae]. One major compound was identified as astragalin [kampferol-3-O-glucoside], and a minor compound that was tentatively identified as quercetin-3-glycoside. The antimicrobial activities of the 2 compounds were tested against 12 microorganisms [7 bacteria, 2 fungi and 3 C and ida of different strains]. The major compound was found to possess a pronounced antibacterial activity as presented by the minimum inhibitory concentration showing activity 4 times as that of streptomycin and double that of tobramycin against Pseudomonas aeruginosa UM 60690. It displayed double activity of streptomycin against both Salmonella typhi UM 26049 and Flavobacterium meningosepticum UM 260494. In addition, the isolated astragalin showed a significant 2-4 times antic and idal activity as compared with nystatin against C and ida intermedia ATCC 5159, C and ida albicans Um 050494, and C and ida lipolytica ATCC 8661. However, this compound did not show any significant antifungal activity. The isolated minor flavonoid showed significant antic and idal, antifungal as well as moderate antibacterial activity

comparative macro- and micromorphology of the leaves, stems and stems and seeds of Vicia sative 1, Vicia calcarate desf and Vicia faba L, [var Minor] growing in Egypt

A macro- and micromorphology of the leaves, stems and seeds of Vicia sativa L., V. calcarata Desf, and V. faba L. [var. Minor] growing in Egypt are presented for the sake of their identification and differentiation in both entire and powdered forms

Macro and micromorphology of leaves, stems and roots of plantago psyllium L growing in Egypt

A macro- and micromorphology of the leaves, stems and roots of Plantago psyllium L. growing in Egypt are presented

Macro and micromorphology of ipomoea carnea jaq growing in Egypt part III: fruit and seed

A study of the macro- and micromorphological characters of the fruit and seed of Ipomoea carnea Jacq. is given in both entire and powdered forms. Many plants belonging to the family Convolvulaceae are known to be useful as purgative drugs. Ipomoea carnea Jacq. is perennial, erect shrub, indigenous to Belgium and was introduced to Egypt for ornamental purpose. Nothing was reported in the current literature concerning both chemical and botanical studies of the plant. The chemical investigation of the resin contents of the plant was recently reported. Meanwhile, the botanical studies of the leaves and flowers, stem and roots were reported, so it was found to be interesting to investigate the botanical characters of the fruits and seeds

Impact of hepatitis C virus [HCV] seropositivity on the serum protein electrophoretic pattern

The impact of HCV seropositivity on the serum protein electrophoretic pattern was analyzed. Abnormalities were tested for prediction of hepatic dysfunction. Sera were obtained from 20 asymptomatic patients seropositive for the virus and 20 control subjects. Sera were subjected to further testing of HCV seropositivity using the second generation enzyme immunonassay kit, and the pattern of binding of anti-HCV antibodies to HCV structural [C1 and C2] and non structural [NS3 and NS4] antigens was determined using the immunoblotting technique. The serum protein electrophoretic pattern was determined and the levels of the different fractions were tested for correlation to the levels of the liver enzymes, serum level of immunoglobulins and to the pattern of reactivity to structural and non structural HCV antigents. The results suggested the importance of the hypergammaglobulinemia as a marker of hepatic dysfunction in HCV seropositive patients, its level might be of value in the follow up of patients with normal liver transaminases levels

T-Cell phenotypes and PHA blastogenic response in symptomatic and asymptomatic Giardia SIS

To determine the relation and the possible significance of peripheral blood T-cell subsets and PHA blastogenesis to various clinical manifestations of Giardiasis, 20 symptomatic, 27 asymptomatic and 18 apparently healthy individuals of matched age and sex were included in this study. Functional analysis of peripheral blood mononuclear cells of asymptomatic Giardia lambia-infection as measured by 3H-thymidine uptake in response to PHA mitogen was significantly inhibited as compared to symptomatic cases of Giardiasis and reference subject groups. On the other hand, phenotypic analysis of the T-lymphocyte subsets showed that CD4+, CD8+, as well as CD4+/CD8+ ratio of asymptomatic, symptomtatic Giardiasis and control groups to be statistically not different from each other. The results suggested a possible role of cell mediated immunity in clearing Giardia lamblia- infection, i.e. development of asymtomatic cases. Meanwhile, the results of the present work showed a defective correlation between peripheral T-lymphocyte subsets and clinical manifestations, i.e. asymptomatic Giardiasis

Phenylpropanoid glycosides from orobanche aegyptiaca and orobanche ramosa

Four phenyl propanoid glycosides, acteoside, 2'-0-acetyl acteoside, poliumoside and 2'-0-acetyl poliumoside were isolated from the butanol extract of Orobanche aegyptiaca Pers. and poliumoside was isolated from the butanol extract of O. ramosa L. The chemotaxonomy of these species was discussed

Essential oils of Thuja occidentalis, Thuja orientalis, Cupressus sempervirens and Juniperus phoenicea

Essential oils were obtained by steam distillation from the twigs and berries of Thuja occidentalis [0.25 and 0.5% w/w respectively], Thuja orientalis [0.32 and 0.25%,w/w], Cupressus sempervirens [0.4 and 0.16 w/w], and Juniperus phoenicea [0.75 and 1.25% w/w]. Comparisons of the four essential oils were made by gas chromatography/mass spectral [GC/MS] analysis. Major constituents of the oils, alpha and were alpha-thujene, [+] sabinene B-pinenes, [+]-camphene, cedrol and cedrenol

Some phenylpropanoid glycosides from Orobanche crenata forsook

A new glycoside, decaffeoylcrenatoside and a known phenylpropanoid glycoside, cistanoside F, have been isolated from the butanol extract of Orobanche crenata Forssk. Crenatoside and acteoside have been identified by TLC. The structures of descaffeoylcrenatoside as well as the known one cistanoside F were established through chemical and spectroscopical methods

Investigation of the lipid and flavonoid content of some orobanche species

Chemical investigation of the petroleum ether and ether extracts of Orobanche aegyptiaca Pers., O. crenata Frossk. and O. ramosa L. afforded a hydrocarbon, a mixture of beta sitosterol, stigmasterol and compesterol, beta sitosterol-3-D-glucoside, tricin, apigenin and luteolin. The fatty acid composition of these plants have been investigated. The chemotaxonomic value of the chemical composition of these extracts and the fatty acid composition have been discussed

Macro-and micromorphology of Veronica anagallis-aquatic var, anagalloides schinzethel

The macro-and micromorphological characters of the different parts of veronica anagallis-aquatica var. anagalloides Schinz et Thell. viz; leaf, stem, flower and root are presented aiming for their identification in both the entire and powdered forms

Macro-and micromorphological study of calendula arvensts L grown in Egypt II the inflorescence

The macro- and micromorphological characters of the inflorescence of Calendula arvensis L. [Family Compositae] grown in Egypt were presented

Phytochemical study of two plantago species: part I: sterols and flavonoids

The chemical investigation of Plantago major L. and P. lagopus L. was carried out. Oleanolic acid, beta sitosterol, campesterol and stigmasterol were isolated from the light petroleum extract. Luteolin and 6-hydroxy luteolin were isolated from the ether fraction of both plants. While the ethyl acetate fraction of P. major L. afforded three phenolic glycosides, viz; acteoside, luteolin-7-O-glucoside and 6-hydroxy luteolin-7-O-glucoside, that of P. lagopus L. afforded three 7-O-arabinosides of apigenin, luteolin and 6-hydroxy luteolin. The chemotaxonomic significance of the isolated arabinosides from P. lagopus L. was discussed

Phytochemical study of two plantago species: part II: iridoid glucosides

The isolation of aucubin, plantarenaloside, ixoroside, asperuloside and melampyroside from Plantago major L. and aucubin, plantrenaloside and asperuloside from Plantago lagopus L. [plantagenaceae] is reported. The identification of the isolated iridoid glucosides was carried out by spectroscopic analysis and comparison with authentic materials

Investigation of the protein content of certain vicia species growing in Egypt

Qualitative and quantitative studies were carried out on the proteins of the stems, leaves and seeds of Vicia faba L. [var. minor], Vicia sativa L. and Vicia calcarata Desf. These studies revealed that the plants contain a large content of nutritive proteins which include high percentage of essential amino acids in their molecules

comparative macro-and micromorphology of plantago major L and plantago lagopus L Part I the leaf, the rhizome and the root

The macro-and micromorphological characters of the leaves, rhizomes and roots of Plantago major L. and P. lagopus L. family Plantaginaceae growing in Egypt are presented

comparative macro-and micromorphology of plantago major L and plantago lagopus L Part II the flower

The macro-and micromorphological characters of the flowers of Plantago major L. and Plantago lagopus L. [Plantagenaceae] growing in Egypt are presented

Serological markers of hepatitis A and B in Bedouin population of north Sinai, Egypt

A total of 254 sera from bedouin subjects in different age groups, located at Rabaa District of North Sinai, were tested by reversed haemagglutination for the existence of hepatitis B surface antigen [HBsAg] in their sera and by enzyme linked immunosorbent assay for serum antibodies to hepatitis B core antigen [anti-HAV]. 100% of the adult population was found to have anti-HAV with antibody acquisition beginning in early childhood and reaching a level of 90% by the age of 10 years. On the other h and, examination of serum specimens for hepatitis B markers showed an overall exposure rate of 22.4%. Anti- HBc [21%] was more detected than anti-HBs [12%]. The relative frequency of the two antibodies varied considerably from group to group. Adult aged group [31 - 40 years] showed the highest exposure while young age group [4 - 10 years] showed the least. Despite these high antibody prevalences, HBsAg was only detected in 3 cases of the study population. These data represented the sero- epidemiological profile of the population prior to introduction of Nile water to the area

Investigation of anthocyanin content of petals of ipoemea carne L growing in Egypt

Five anthocyanin pigments were isolated from petals of flowers of Ipoemea carne L. [Family Convolvulaceae] and identified by their chromatographic, spectral and chemical properties. This study revealed the presence of cyanidin 3 B-rhamnoside, cyanidin 3,5 B-diglucoside, petunidin 3 B-galactoside, peonidin 3 B-arabinoside and pelargonidin 3 B-sophoroside 7 B-glucoside