Résumé
Penicillin resistance plasmid was transferred from Staphylococcus aureus B4 (PcrKms, donor) to S. aureus ML351 (PcsKmr, recipient) by co-cultivation of the donor with the recipient in nutrient broth with or without the modifying effects of CaCl2 or sodium dodecyl sulfate. It was found that the transfer of drug-resistance occurred maximally between 6 and 18 hr postinoculation; however, addition of DNase (200 micrograms/ml) could totally prevent such a transfer up to 6 hr and significantly reduce it thereafter. Cell-free filtrate of the donor culture when mixed with the recipient was ineffective in bringing about the transfer of Pcr.
Sujets)
Conjugaison génétique , Techniques de transfert de gènes , Résistance à la kanamycine/génétique , Résistance aux pénicillines/génétique , Plasmides , Phages de Staphylococcus/génétique , Staphylococcus aureus/effets des médicaments et des substances chimiquesRésumé
Outbreak of Sarcoptes scabiei in animals spilling over to man in close association was observed in two adjacent villages, Fewgram and Nurpur in the district of Birbhum, West Bengal, from mid-November to mid-December, 1991. Nineteen goats and one calf who did not receive any treatment died of sarcoptic manage. All infected animals got cured with external application of deltamethrin, a synthetic pyrethroid and triazapentadiene. Human cases were treated successfully with benzene hexachloride (2%).
Sujets)
Adulte , Animaux , Bovins , Enfant , Épidémies de maladies , Chiens , Femelle , Capra , Humains , Inde/épidémiologie , Insecticides/usage thérapeutique , Lindane/usage thérapeutique , Mâle , Nitriles , Pyréthrines/usage thérapeutique , Gale/traitement médicamenteux , Ovis , ZoonosesRésumé
Proven cases (28) of glomerulonephritis (GN) were evaluated for clinico-biochemical profile, bleeding time, platelet count, platelet aggregation and platelet serotonergic mechanisms. Inordinate increase in platelet aggregation and altered platelet serotonergic mechanisms (reduced 5-HT, uptake, reduced intraplatelet 5-HT and increased plasma 5-HT) were demonstrated in some histopathological types of GN. The mechanisms and importance of these alterations have been discussed.