Résumé
Abstract The aim of this study is to evaluate the expression of cytokines in response to mineral trioxide aggregate (MTA) plus selenium in germ-free mice with experimental furcal perforation. The first left maxillary molar was opened, and the furcal area was perforated and treated with post-MTA-Se (experimental group). The same surgical intervention was performed for the maxillary right first molar, which was treated with MTA (control group). Fifteen mice were sacrificed 7, 14, and 21 days after furcal perforation, and periapical tissue samples were collected. The mRNA expression levels of the cytokines TGF-β, TNF-α, IFN-γ, HPRT, IL-10, IL-4, RANK, RANKL, IL-1, and IL-17 were assessed by using real-time polymerase chain reaction. In the experimental group, at 21-days post-MTA-Se sealing, the mRNA levels of TNF-α and IL-10 were upregulated compared with those in the control group (p < 0.05). Futher assessment revealed basal mRNA expression levels of IL-1α, IFN-γ, RANK, RANKL, IL-17A, IL-4, and TGF-β, over long experimental times, in both the experimental and control groups (p > 0.05). In conclusion, MTA+Se sealing favoured increased expression of IL-10 and TNF-α at later time points (day 21).
Sujets)
Animaux , Mâle , Femelle , Oxydes/pharmacologie , Produits d'obturation des canaux radiculaires/pharmacologie , Sélénium/pharmacologie , Cytokines/analyse , Silicates/pharmacologie , Anomalies de furcation/traitement médicamenteux , Composés du calcium/pharmacologie , Composés de l'aluminium/pharmacologie , Cavité pulpaire de la dent/traumatismes , Traitement de canal radiculaire/méthodes , Facteurs temps , Reproductibilité des résultats , Résultat thérapeutique , Anomalies de furcation/immunologie , Cavité pulpaire de la dent/effets des médicaments et des substances chimiques , Cavité pulpaire de la dent/immunologie , Association médicamenteuse , Réaction de polymérisation en chaine en temps réel , Molaire/effets des médicaments et des substances chimiques , Molaire/traumatismesRésumé
A infecção dos sistemas de canais radiculares levará à consequente indução de uma lesão periapical, estando muitos mediadores inflamatórios envolvidos nesse processo. A IL-17 tem demonstrado impacto no processo de remodelação óssea, especialmente em casos de artrite, apresentando-se em altos níveis. Contudo, pouco se sabe a respeito do seu real papel na patogênese das periapicopatias. Neste estudo, procurou-se avaliar o papel da IL-17A na reabsorção óssea perirradicular, em infecções endodônticas experimentais em camundongos C57BL/6 e IL-17RA KO, bem como o efeito dessa infecção na artrite reumatóide experimental. Para se induzir a infecção endodôntica, cepas padrão de Porphyromonas gingivalis, Prevotella intermedia e Fusobacterium nucleatum foram inoculadas nos canais radiculares do primeiro molar superior esquerdo desses animais...