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Indian J Exp Biol ; 2007 Aug; 45(8): 669-75
Article Dans Anglais | IMSEAR | ID: sea-60809

Résumé

The tumor necrosis factor-alpha (TNF-alpha) plays an important role in ovarian follicular development and ovulation process and acts through its receptor (TNFRI). The present investigation describes the expression of mRNAs encoding TNF-alpha and TNFRI in relation to glyceraldehyde-3-phosphate dehydrogenase (G3PDH) and beta-actin as control genes, using RT-PCR, in granulosa cells, intact follicles and luteal tissues from buffalo ovary. There was significant higher expression of mRNAs encoding TNF-alpha in granulosa cells from medium follicles and TNFRI expression increased with increase in size of follicles. Post-ovulatory structures (corpus luteum and corpus albicans) exhibited significantly higher expression of TNFRI mRNAs as compared to that obtained in intact follicles suggesting its immediate and critical role just after ovulation, for mediating TNF-alpha action on these tissues. Though the expression of TNF-alpha mRNA was stimulated by treatment of granulosa cells with FSH during culture, the expression of TNFRI mRNA did not change. The FSH alongwith IGF-I did not exert any effect. These results suggested an important role of TNF-alpha and its receptor in buffalo ovarian functions.


Sujets)
Actines/génétique , Animaux , Buffles/croissance et développement , Corps jaune/métabolisme , Femelle , Hormone folliculostimulante/pharmacologie , Glyceraldehyde 3-phosphate dehydrogenases/génétique , Cellules de la granulosa/effets des médicaments et des substances chimiques , Facteur de croissance IGF-I/pharmacologie , Follicule ovarique/effets des médicaments et des substances chimiques , Ovaire/effets des médicaments et des substances chimiques , ARN messager/analyse , Récepteur au facteur de nécrose tumorale de type I/génétique , Facteur de nécrose tumorale alpha/génétique
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