Résumé
Aims:To report estimates of the reliability and agreement of a new method for measuring the femoral Neck-shaft angle in the Jordanian population. The neck-shaft angle is an important anatomical indicator in orthopedics of the hip. While there are different approaches to measuring the neck-shaft angle in the literature, there is no agreement on the best technique used for measurement. CT scout view was used in this study to provide a promising alternative. Study Design: Observational reliability and agreement study.Places and Duration of the Study:Department of physiotherapy, school of rehabilitation science, University of Jordan and University of Jordan Hospital between March 2014 and October 2015. Methodology:Two independent raters calculated the neck-shaftangle on each hip of 50 pelvic CT scout images of healthy adults to determine inter-rater reliability. One rater performed the measurement twice to determine the intra-rater reliability. Intra-class correlation coefficients were used to examine relative reliability. The standard error of measurement (SEM) and 95% minimal detectable change (MDC) were calculated to examine absolute reliability. Results:The mean value of all angle measurements was 131.3. Intra-class correlation coefficients were 0.726 and 0.63 for inter and intra-rater measurements respectively. SEM and MDC for inter-rater measurements were 2.69 and 7.46 respectively. For intra-rater measurements, they were 2.84 and 7.86 respectively. Conclusion: The new method proposed in this study for measuring the neck-shaft angle showed good reliability and small measurement error.
Résumé
Aim of the Study: Both apoptotic induction and cell cycle blockade in cancer cells are effective strategies to eliminate cancer cells. Many conventional cancer drugs that induce apoptosis and inhibit cell cycle progression have been reported as potential therapeutics for various types of cancer. Britannin is a natural sesquiterpene lactone that its profound anticancer properties were revealed in our previous study. In this study, we evaluated the effects of britannin on the cell cycle distribution and also cell cycle-related proteins. Materials and Methods: Analysis of cell cycle distribution was carried out using flow cytometer. The effects of britannin on cyclin D1 and CDK4 expression were evaluated using the Western blot. Results: The obtained results show that britannin at the low concentrations induces cell growth inhibition mainly through G1-phase arrest while it seems that apoptosis contributes to cell growth inhibitory effect of high doses of britannin. Reduction of cyclin D1 and CDK4 protein levels were also observed after treating cancer cells with britannin. Conclusion: The obtained results reveal that britannin can inhibit MCF-7 and MDA-MB-468 breast cancer cells proliferation through arresting cell cycle progression through cyclin D1/CDK4-mediated pathway