Ultrastructural changes in the corpus luteum of Egyptian buffalo-cows during different phases of the oestrous cycle

In this work, the relationship between histological structure of corpus luteum and steroid hormones during estrous cycle of buffalo- cows was studied. Corpora lutea and blood samples were collected from slaughtered animals. The corpora lutea were prepared for a histological examination using electron microscope, while progesterone and estradiol-17 beta were estimated from serum. The results revealed a relation between fine structure of lutein cells and concentrations of progesterone during the same stage of the estrous cycle. The main change which occurred in the cytoplasm of the lutein cells was variation of the number of the secretory granules. After metestrus phase and during the diestrus one, levels of progesterone increased gradually and the cytoplasm of the lutein cells became vacuolated containing lipid droplets, electron dense granules, numerous mitochondria and SER. At estrus phase, a significant sharp decrease of progesterone and a rise of estradiol-17 beta were associated with severe changes in luteal cells of corpus albicans containing numerous lipid droplets of different sizes and empty spaces, followed by a disintegration of the cytoplasm which became cornified

investigation of the possible mechanism for propranolol induced sexual dysfunction

Propranolol as a beta adrenergic blocking agent is widely used in therapeutics for many indications. The drug has been observed to produce sexual dysfunction in many patients. However, the mechanisms of this effect is still unclarified. The present work is designed to investigate the effect of subchronic administration of propranolol on rat testicular tissues and serum testosterone level. Propranolol was given in 3-dose levels for 4 weeks. Results revealed that propranolol could produce degenerative changes in the interstitial cells of Leydig as well as the sperm cells and this associated with significant reduction in serum testosterone level. These changes may be the cause of sexual dysfunction that complicate propranolol therapy. The possible mechanisms underlying these changes were discussed

effect of repeated morphine adminstration on the structure of lung in albino mice: a light and scanning electron microscopic study

In this study, specimens of lungs of both control and morphine injected male mice were examined using light and scanning electron microscope. The lungs of treated animals showed focal histological changes in the form of narrowing of lung alveoli, degeneration of alveolar epithelium and thickening of interalveolar septa. The blood vessels among the alveoli showed hyaline thickening of their walls, dilation and congestion. Histiocytes containing variable materials and vacuoles were seen in the thickened interalveolar septa and among cellular infiltration. The larger pulmonary blood vessels showed emboli. Light microscopic lung features were confirmed and three dimensionally described using scanning electron microscope. The results were discussed in view of the available literature in a trial to explain the frequency of occurrence of pulmonary hypertension and respiratory failure associated with drug addiction

Effects of a mixture of progesterone and estrogen on the histology of the ovary and oviduct of lactating albino rat

Administration of a mixture of progesterone and estrogen to lactating albino rats revealed persistence of different stages of follicles. Most of these follicles underwent degeneration. Hyperlasia of ovarian stromal cells was also noticed. The oviducts revealed proliferation of the lining epithelium