Résumé
This work was done to study the effects of exposure to mobile emits 900MHz electromagnetic field [EMW] on the uterus and ovaries of female rats. Thirty female adult rates were randomly divided into three groups [10 each] as follows: negative control group [1], positive control group [II]: without exposure to electromagnetic wave [exposure device off for 30 min/day for 30 days]. Exposure group [III]: exposed to 900MHz electromagnetic wave [EMW] for 30 min/day for 30 days. The results showed a significant reduction in ovarian weights and non significant change in uterine weight in the exposed rats [group III] when compared to the other control groups. The Serum levels of estrogen and progesterone were significantly decreased among EMW exposed group. Ovarian and endometrial tissue homogenate revealed a significant increase in malondialgehyde [MDA] levels, while they showed a significant decrease in the activity of the reduced gluthathione [GSH] in the same group. In the EMW exposed group, histopathological changes revealed many apoptotic cells with densely stained cytoplasm and fragmented or phyknotic nuclei, in the endometrial surface, epithelial and glandular cells. The glands were significantly decreased in number and diffuse inflammatory cell infiltration were observed in the endometrial stroma. Sections of ovaries revealed significant decrease in the follicle numbers in EMW exposed group III compared to both control groups. Uterine sections immunolabeled for active caspase-3, showed significant increasing in numbers of immunolabeled cells for activated caspase 3 and apoptotic cells, in EMW-exposed group III. In conclusion, this study demonstrated that there is impairment in endometrial and ovarian tissues both at biochemical and histological levels after experimental exposure to 900-MHz emitted mobile phone