Résumé
The functional characterisation of thousands of
Sujets)
Vecteurs génétiques/génétique , Trypanosoma cruzi/génétique , Chromatographie d'affinité , Clonage moléculaire , Étiquettes de séquences exprimées/métabolisme , Expression des gènes/génétique , PlasmidesRésumé
Calpains are calcium-dependent cysteine proteinases found in all living organisms and are involved in diverse cellular processes. Calpain-like proteins have been reported after in silico analysis of the Tritryps genome and are believed to play important roles in cell functions of trypanosomatids. We describe the characterization of a member of this family, which is differentially expressed during the life-cycle of Trypanosoma cruzi.