Cytogenetic and FMS-like tyrosine kinase 3 mutation analyses in acute promyelocytic leukemia patients

The secondary genetic changes other than the promyelocytic leukemia-retinoic acid receptor [PML-RARA] fusion gene may contribute to the acute promyelocytic leukemogenesis. Chromosomal alterations and mutation of FLT3 [FMS-like tyrosine kinase 3] tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia. However, the prognostic significance of FLT3 mutations in acute promyelocytic leukemia [APL] is not firmly established. In this study, the chromosomal abnormalities were analyzed by bone marrow cytogenetic in 45 APL patients and FLT3 internal tandem duplications [ITD] screening by fragment length analysis and FLT3 D835 mutation by melting curve analysis were screened in 23 APL samples. Cytogenetic study showed 14.3% trisomy 8 and 17.1% chromosomal abnormalities other than t[15;17]. About 13% of the patients had FLT3 ITD, and 26% had D835 point mutation. FLT3 ITD mutation was associated with higher white blood cell count at presentation and poor prognosis. The PML-RARA translocation alone may not be sufficient to induce leukemia. Therefore, we assume that FLT3 mutations and the other genetic and chromosomal alterations may cooperate with PML-RARA in the development of APL disease

[ effect of blood storage on apoptosis induction of Jurkat cells: an in vitro model]

It is a firm belief that blood transfusion is life-saving in many situations, but at the same time transfusion complications could be life-threatening. The possible effects of blood Transfusion Related Immunomodulatory [TRIM] and its related mechanisms is one of the important debatable subjects in the field of blood transfusion medicine. One of the mechanisms through which transfusion can induced TRIM effects in recipient is apoptosis induction. Aim of this study was to investigate the apoptotic effects of stored blood in an in vitro model. To evaluate the apoptotic effects of blood storage, we studied the effect of the plasma [from whole blood] during storage on days 3, 10, 21 and 35 on Jurkat cells, which are sensitive to apoptosis .The plasma of whole blood was separated by centrifugation on different days. Then, Jurkat cells were cultured with plasma for 24 hours. Finally apoptosis level was studied by using flowcytometry for analyzing Annexin V on Jurkat cells [by SeroTec Annexin V:FITC Assay Kit]. The percentages of apoptosis on days 3, 10, 21 and 35 were 3.85 +/- 1.52, 5.27 +/- 2.12, 8.44 +/- 1.90, 12.01 +/- 2.32, respectively. The percentages of apoptotic cells in negative and positive control group was 3.85 +/- 1.94 and 65.80 +/- 2.28, respectively. The results of this study showed that plasma of whole blood have the apoptotic effects which enhanced during the storage of plasma. This in vitro model is also suitable for studying other TRIM related mechanisms