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1.
Braz. j. med. biol. res ; 27(3): 601-11, Mar. 1994. ilus, graf
Article Dans Anglais | LILACS | ID: lil-148932

Résumé

1. After MHV3 infection, only macrophages from resistant A/J mice partially restricted virus growth compared to those from susceptible BALB/c mice (2 logs of difference in virus titer). 2. Cellular ribosomal ribonucleic acid (rRNA) synthesis by MHV3-infected macrophages was decreased only in A/J mouse macrophages as indicated by accumulation of the 28S rRNA fraction. 3. The accumulation of viral messenger ribonucleic acids (mRNAs) in MHV3-infected macrophages was also reduced in A/J mouse macrophages compared to BALB/c mice. 4. In pulse-chase experiments of viral protein synthesis, the appearance, glycosylation and cleavage of glycoprotein S, as well as the metabolism of nucleoprotein N were delayed in A/J mouse macrophages. 5. These data show that MHV3 infection of A/J mouse macrophages induced an imbalanced accumulation of the 28S fraction of rRNA. Furthermore the synthesis of mRNAs correlated with viral protein synthesis in both A/J and BALB/c macrophages, but was delayed in A/J mice. 6. These results suggest that the partial restriction of MHV3 replication in macrophages of resistant A/J mice may take place during or before the mRNA synthesis, although it is correlated with the appearance, glycosylation, cleavage and metabolism of viral proteins


Sujets)
Humains , Souris , Hépatite virale animale/métabolisme , Infections à coronavirus/microbiologie , Macrophages/microbiologie , ARN ribosomique/biosynthèse , ARN messager/biosynthèse , ARN viral/biosynthèse , Virus de l'hépatite murine/physiologie , Macrophages/métabolisme , Souris de lignée A , Souris de lignée BALB C , Facteurs temps , Réplication virale
2.
Braz. j. med. biol. res ; 26(5): 509-18, May 1993. tab, graf
Article Dans Anglais | LILACS | ID: lil-148705

Résumé

1. After immunization, adult A/J mice are resistant and BALB/c mice are susceptible to MHV3 infection. After IFN gamma activation, only macrophages originating from A/J mice were able to partially restrict MHV3 growth. 2. When the binding of MHV3 and interferon (IFN) gamma to solubilized cytoplasmic and membrane macrophage proteins of mice was determined by ELISA, there was more binding of MHV3 to proteins extracted from BALB/c macrophages than to proteins extracted from A/J macrophages. When the proteins were obtained from IFN gamma-activated macrophages, decreased MHV3 binding was observed only in proteins originating from A/J macrophages. 3. ELISA showed a comparable binding of IFN gamma to A/J or BALB/c macrophage proteins. When the proteins were obtained from IFN gamma-activated macrophages, only IFN gamma-binding to A/J macrophage proteins was increased. 4. The results indicate a different expression and IFN gamma modulation of MHV3 receptors in macrophages from A/J and BALB/c mice, which directly correlated with their acquired resistance or susceptibility to MHV3 infection


Sujets)
Animaux , Souris , Hépatite virale animale/immunologie , Immunisation , Interféron gamma/pharmacologie , Macrophages/métabolisme , Virus de l'hépatite murine/croissance et développement , Test ELISA , Protéines membranaires/métabolisme , Souris de lignée A , Souris de lignée BALB C
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