Healing potential of mesenchymal stem cells cultured on a collagen-based scaffold for skin regeneration

Wound healing of burned skin remains a major goal in public health. Previous reports showed that the bone marrow stem cells were potent in keratinization and vascularization of full thickness skin wounds. In this study, mesenchymal stem cells were derived from rat adipose tissues and characterized by flowcytometry. Staining methods were used to evaluate their differentiation ability. A collagen-chitosan scaffold was prepared by freeze-drying method and crosslinked by carbodiimide-based crosslinker. The results of immunecytochemistry and PCR experiments confirmed the adipose-derived stem cells [ASC] in differentiation to the keratinocytes under the treatment of keratinocyte growth factor. The isolated ASC were seeded on the scaffolds and implanted at the prepared wounds. The scaffolds without cells were considered as a control and implanted on the other side of the rat. Histopathological analyses confirmed the formation of new tissue on the scaffold-cell side after 14 days with the formation of dermis and epidermis. These results indicated the capacity of ASC in differentiation to keratinocytes and also wound healing in vivo

[Schizophrenia and ventricular system]

Background: Regarding to the limited clinical possibilities for schizophrenia diagnosis and treatment and the importance of cerebrospinal fluid in it seems study of the relative structures of ventricular system can lead us to the new diagnostic points

Materials and Methods: For this review article we have used through Pub. Med, Yahoo and Google sites following keywords schizophrenia-ventricular system-lat ventricle -third ventricle -fourth ventricle -sylvian canal-cerebrospinal fluid-mesencephalic duct-caudate nucleus-amygdaloid between 1 99Oand 2001 years. The study was focused on anatomical findings

Results: In the related structures of ventricular systems cytological changes in thalamus , basal ganglia and hipochampus, fractional anizotropy induction in corpus callosum, cerebellar supersensitivity was found

Conclusion: Regard to recent new findings of periventricular spaces theses anatomical focuses may be useful for new research on diagnosis and treatment of shizophrenia

[Schizophrenia and ventricular system]

Backgroud: Regarding to the limited clinical possibilities for schizophrenia diagnosis and treatment and the importance of cerebrospinal fluid in it, it seems study of the relative structures of ventricular system can lead us to the new diagnostic points

Materials and Methods: For this review article we have used through Pub. Med, Yahoo and Google sites following keywords: schizophrenia-ventricular system-lat ventricle -third ventricle -fourth ventricle -sylvian canal-cerebrospinal fluid-mesencephalic duct-caudate nucleus-amygdaloid between 1990 and 2001 years. The study was focused on anatomical findings

Results: In the related structures of ventricular systems cytological changes in thalamus , basal ganglia and hipochampus, fractional anizotropy induction in corpus callosum, cerebellar supersensitivity was found

Conclusion: Regard to recent new findings of periventricular spaces theses anatomical focuses may be useful for new research on diagnosis and treatment of shizophrenia