Résumé
A study was carried out using macrophages cultured from the peritoneal exudate of dogs infected in vitro with three species of Leishmania: L. (L.) chagasi, L. (Viannia) braziliensis and L. (L.) amazonensis with the aim of investigating the growth kinetics and infectivity of these species in the host cell. Results were expressed as the percentage of macrophages infected measured at 24 hr intervals over six days in RPMI - 1640 culture medium at a temperature of 34-35oC. The findings open the possibility of using canine peritoneal cells as a model for the screenning of leishmanicide drugs and to study the pathogenesis of these species.
Sujets)
Animaux , Chiens , Mâle , Femelle , Techniques in vitro , Leishmania/pathogénicité , Macrophages péritonéaux/parasitologie , Leishmania brasiliensis/pathogénicité , Leishmania infantum/pathogénicité , Leishmania mexicana/pathogénicité , Leishmaniose cutanée , Leishmaniose cutanéomuqueuse , Leishmaniose viscéraleRésumé
Cutaneous disseminated lesions caused by Leishmania sp. were found in a pregnant mare (Equus cabalus) from a rural city in the State of rio de Janeiro, Brazil. Before delivering, treatment was undertaken by immunotherapy followed by chemotherapy. Histopatology and serology were performed during treatment, as well as the biochemical characterization of the parasite (L. braziliensis) that was isolated from one of the lesions
Sujets)
Animaux , Femelle , Grossesse , Complications parasitaires de la grossesse/médecine vétérinaire , Maladies des chevaux/parasitologie , Leishmania brasiliensis , Leishmaniose cutanée diffuse/médecine vétérinaire , Complications parasitaires de la grossesse/thérapie , Maladies des chevaux/thérapie , Equus caballus , Immunothérapie , Leishmaniose cutanée diffuse/thérapieRésumé
In order to characterize the epidemiology of American Cutaneous Leishmaniasis (ACL) in a periurban area of the municipality of Sabará in the metropolitan region of Belo Horizonte (MRBH), an area until then considered free of the disease, a cross sectional survey was undertaken in 1990. The survey of the population consisted of 1119 interviews and 881 clinical examinations using Montenegro's skin test (MST). A low prevalence (3.7%) of positive MST was encountered. The disease had been occuring in the area for about 20 years in the form of sporadic cases. The predominant species of sandfly both in domestic areas and nearby areas of secondary vegetation was Lutzomyia whitmani. A canine survey of delayed hypersensitivity to the antigen P10,000 identified only one dog with a positive reaction out of 113 examined. The transmission of ACL in MRBH was confirmed. The occurrence of the disease in women, children and individuals with no contact with forest areas as well as the presence of potential vector species in the domiciliar environment, suggests the transmission of the disease in this environmewnt