Short-term creatine supplementation changes protein metabolism signaling in hindlimb suspension

The effect of a short-term creatine supplementation on hindlimb suspension (HS)-induced muscle atrophy was investigated. Creatine monohydrate (5 g/kg b.w. per day) or placebo, divided in 2 daily doses, was given by oral gavage for 5 days. Rats were maintained in HS with dietary supplementation concomitantly for 5 days. Body weight, soleus and EDL muscle masses, and cross-sectional areas (CSA) of the muscle fibers were measured. Signaling pathways associated with skeletal muscle mass regulation (FST, MSTN, FAK, IGF-1, MGF, Akt, mTOR, atrogin-1, and MuRF1 expressions, and Akt, S6, GSK3B, and 4EBP1 proteins) were evaluated in the muscles. Soleus muscle exhibited more atrophy than the EDL muscle due to HS. Creatine supplementation attenuated the decrease of wet weight and increased p-4EBP1 protein in the EDL muscle of HS rats. Also, creatine increased mTOR and atrogin-1 expressions in the same muscle and condition. In the absence of HS, creatine supplementation increased FAK and decreased MGF expressions in the EDL muscle. Creatine attenuated the increase in FST expression due to HS in the soleus muscle. MuRF1 expression increased in the soleus muscle due to creatine supplementation in HS animals whereas atrogin-1 expression increased still further in this group compared with untreated HS rats. In conclusion, short-term creatine supplementation changed protein metabolism signaling in soleus and EDL muscles. However, creatine supplementation only slightly attenuated the mass loss of both muscles and did not prevent the CSA reduction and muscle strength decrease induced by HS for 5 days.

Revisión sistemática de la efectividad funcional del vendaje neuromuscular en sujetos con inestabilidad de tobillo / A systematic review of the functional effectiveness of kinesiotaping in individuals with ankle instability

INTRODUCCIÓN La inestabilidad de tobillo presenta mayor frecuencia en personas que han sufrido repetidos esguinces de tobillo. Aunque el Kinesio Tape es una técnica masificada en los últimos años en el tratamiento de esta lesión, se desconoce su efectividad. OBJETIVO Determinar la efectividad funcional del Kinesio Tape en individuos con inestabilidad de tobillo. DISEÑO Revisión sistemática. FUENTES DE DATOS Se realizó una búsqueda sistemática de artículos en donde se aplicó Kinesio Tape en sujetos con inestabilidad de tobillo en las siguientes bases de datos: PubMed Central; PubMed/Medline; Cochrane; Embase; Sciencedirect; Biomed Central; CINAHL; Lippincott Williams &Wilkins; SAGE y Wiley-Blackwell. MÉTODOS DE LA REVISIÓN Se seleccionaron solo estudios experimentales tipo ensayo clínico, en donde se interviniera a pacientes con inestabilidad de tobillo mediante la aplicación de Kinesio Tape y que incluyeran al menos una variable de resultado asociada a la función del tobillo. Se aplicó la escala PEDro a cada artículo para medir su calidad metodológica. RESULTADOS Se obtuvieron 364 artículos. Diez cumplieron los criterios de inclusión; cinco de alta y cinco de moderada calidad metodológica. En la mayoría de los artículos se midió más de una variable. Dos artículos presentaron mejoras en la fuerza muscular; cuatro artículos midieron equilibrio, dos observaron mejoras; un artículo observó mejoras en la propiocepción; dos artículos midieron control postural, sin mejoras significativas y un artículo no observó mejoras significativas en control neuromuscular. Estabilidad articular (un artículo), actividad muscular (un artículo) y funcionalidad (un artículo), no reportaron mejoras significativas con el uso de Kinesio Tape. CONCLUSIÓN El Kinesio Tape es efectivo para obtener mejoras en la fuerza muscular, el equilibrio y la propiocepción en individuos con inestabilidad de tobillo. Sin embargo, el Kinesio tape no reporta mejoras significativas en control postural, control neuromuscular, actividad muscular, estabilidad articular y funcionalidad en sujetos con inestabilidad de tobillo.

INTRODUCTION Ankle instability often seen in people who have suffered repeated ankle sprains. Kinesio Tape is a technique widely applied in recent years, despite not having enough evidence to support its application. OBJECTIVE To determine the functional effectiveness of Kinesio Tape in individuals with ankle instability. DESIGN Systematic review. DATA SOURCES A systematic search of articles was performed where Kinesio Tape was applied in subjects with ankle instability in the following databases: PubMed Central; PubMed/Medline; Cochrane; Embase; Sciencedirect; Biomed Central; CINAHL; Lippincott Williams & Wilkins; SAGE and Wiley-Blackwell. METHODS OF THE REVIEW Only clinical trials were selected, in which patients with ankle instability were intervened by applying Kinesio Tape and which included at least one outcome variable associated with ankle function. RESULTS364 articles were found. Ten met the inclusion criteria; five presented high and five moderate methodological quality. In most cases, one article measured more than one variable. Two articles showed improvements in muscle strength. Of four articles that evaluated balance, two observed improvements; one article observe improvements in proprioception; two articles measured postural control, without significant improvements; one article did not observe significant improvements in neuromuscular control. Stability (one article), muscular activity (one article) and functionality (one article) did not report significant improvements with the use of Kinesio Tape. On the other hand, Kinesio tape does not report significant improvements with respect to postural control, neuromuscular control, muscle activity, joint stability and functionality in subjects suffering from ankle instability. CONCLUSION Kinesio Tape is effective for improvement of muscle strength, balance and proprioception in subjects with ankle instability. However, Kinesio Tape does not report significant improvement of postural control, neuromuscular control, muscle activity, joint stability and functionality in people with ankle instability.