Résumé
Background: Cervical cancer is one of the important reasons of mortality among females. Prevention, early diagnosis and immediate treatment can affect the rate of mortality in this cancer and several epidemiological studies have shown a strong relationship between human papilloma viruses [HPVs] and cervical cancer
Objectives: The present study was conducted to survey HPV infections in a women population with cervical cancer and cervical dysplasia/metaplasia in southwest of Iran
Materials and Methods: 72 paraffin-embedded cervical biopsies which had been previously archived from women with cervical cancer and cervical dysplasia were examined by polymerase chain reaction [PCR]. Afterward, the detected HPV strains were typed by restriction fragment length polymorphism [RFLP] analysis of PCR amplicons
Results: 60 out of 72 samples had necessary requirements and HPV DNA was detected in 43.3% of these samples. Most HPV positive samples belonged to women aged from 48 to 63 years. On the other hand, HPV infection among patients with squamous cell carcinoma [SCC] was 48.78% and in women with dysplasia/metaplasia was 26.66%. The most prevalent type of the human papilloma virus was HPV16 [100%]
Conclusions: Knowing the most prevalent type of the human papilloma viruses circulating in the population [HPV16] can be applied in the future screening and managing programs of this major disease and also in vaccination against the prevalent types of the virus. Meanwhile, it seems that more studies should be performed to determine the role of different risk factors involved in development of the disease, especially those related with social behaviors and traditions with respect to different areas
Résumé
DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gel electrophoresis [PAGE]. DNA markers can be prepared by mixing PCR products with definite sizes. Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages or natural and synthetic DNA plasmids. The present study describes engineering of a synthetic plasmid which produces a 100 bp DNA ladder, a popular DNA size marker, upon digestion with a single restriction enzyme. Our strategy consisted on sequential cloning of ten PCR products of 100 to 1000 bp in plasmid pTZ57R, using the BamHI and Bg/II restriction sites and releasing the fragments from the recombinant plasmid by enzyme EcoRV. This strategy could be applied to construct various complex synthetic vectors to produce different DNA ladders