Investigation of single-strand conformational polymorphism of the TP53 gene in women with a family history of breast cancer

Breast cancer in families with germ line mutations in the TP53 gene has been described in the medical literature. Mutation screening for susceptibility genes should allow effective prophylactic and preventive measures. Using single-strand conformational polymorphism, we screened for mutations in exons 5, 6, 7 and 8 of gene TP53 in the peripheral blood of 8 young non-affected members (17 to 36 years old) of families with a history of breast cancer. Studies of this type on young patients (mean age, 25 years) are very rare in the literature. The identification of these mutations would contribute to genetic counseling of members of families with predisposition to breast cancer. The results obtained did not show any polymorphism indicating mutation. In our sample, the familial tumorigenesis is probably related to other gene etiologies

Morfometria do úbere em caprinos: correlaçäo com produçäo de leite, taxa de ordenha e incidência de mastite / Udder morphometry in goats: correlation between milk production, milking rate and mastitis incidence

The udder measurement of 47 lactating does, in lactation, 22 Nubians, 6 Alpines and 19 Saanens, were studied. The milking time (TE) was measured from the moment attaching machine milking cups on the udder until the end of milking. The milking rate (TA) was defined as PL/TE, where PL is the daily milk production. The least square means for PL, TE, TA and CMT were 1,48ñ39g, 87ñ3s, 17.6ñ0.8g/s and 0.63ñ0.07, respectively. The udder perimeter (PU), teat perimeter (PT) teat length (CT), udder cleft (AL), visual appraisal of udder shape (globular or non-globular), teat shape (ballon, cylinder, funnel or bottle) and teat end shape (round or sharp) were described and their effect on PL, TE, TA and CMT were also studied. Females with sharp teat end sharp had tower incidence of mastitis than female with round teat end round (P<0.05). The PU had a significant effect (P<0.01) on PL, TE and TA. The correlation coefficient between PU and PL, PU and TE, PU and TA were 0.78, 0.51 and 0.41, respectively, and a negative correlation of -0.30 (P<0.05) between PU and CMT. Results suggest the possibility of selection based on the udder morphometry