Cytotoxic activity of the chloroform extract and four diterpenes isolated from Salvia ballotiflora

Abstract New compounds with chemotherapeutic activity are sought after, and plants are an important source of these compounds. Four diterpenes, 19-deoxyicetexone, 7,20-dihydroanastomosine, icetexone and 19-deoxyisoicetexone, were isolated from the hexane-washed chloroform extract of Salvia ballotiflora. The cytotoxic activity of the hexane-washed chloroform extract and its four diterpenes were tested using the MTT assay against three tumor cell lines: HeLa (cervical cancer), A549 (lung cancer) and MCF7 (breast cancer), and two murine cell line: J774A.1 (epithelial cancer) and CT26 (colon cancer), and their IC50 values were determined. 19-Deoxyisoicetexone had the greatest effect on HeLa cells with IC50 of 3.2 µg/ml (9.36 µM), whereas hexane-washed chloroform extract had the best cytotoxic effect on A549 cells with an IC50 of 2.29 µg/ml. These effects of 19-deoxyisoicetexone and hexane-washed chloroform extract were with similar activity compared to cisplatin (IC50 = 1.06 µg/ml in HeLa cells, and 4.6 µg/ml (15.21 µM) in A549 cells).

Doença de Dupuytren: nossa conduta / Dupuytren’s disease: our conduct

Introdução: A doença de Dupuytren é caracterizada pela contratura em flexão progressiva dosdígitos, o que pode levar à incapacidade funcional da mão. O objetivo deste trabalho épreconizar uma técnica operatória eficaz e menos traumática para conduzir a doença. Método:Foram tratados cirurgicamente 55 pacientes em vários estágios da doença. Resultados:Em todos os casos, foram obtidos resultados satisfatórios, com bom retorno dos pacientesàs suas atividades.

Introduction: The Dupuytren’s disease is characterized by the progressive contracture inflexion of the digits, which could carry the hand to a full functional incapacity. The purposeof this study is precognize an efficient operative technique less traumatic for treating thedisease. Methods: Fifty-five patients underwent to surgery, in different levels of the disease,were analyzed. Results: In all of the cases were obtained satisfactory results, returning thepatients to their activities.

Intubación endotraqueal prolongada / Prolonged endotracheal intubation

Se ha desarrollado una tendencia en años recientes hacia el uso de la intubación endotraqueal por períodos prolongados como soporte ventilatorio para el paciente en estado crítico postoperado, antes de realizar la traqueostomía. Numerosos factores han sido implicadas en la etiología de las complicaciones a la intubación endotraqueal prolongada, estas se han disminuido en forma importante con el advenimiento de de las sondas con globos de alto volumen y baja presión. En el presente reporte nosotros estudiamos dos casos de intubación prolongada, revisando las complicaciones a la postextubación y los criterios para facilitar la extubación.