Résumé
Objective: To study the chemical constituents in theendophytic fungus Nigrospora oryzae from Cordyceps. Method: It was cultured with brownrice,and isolated and purified by chromatographic procedures, and the compounds were identified by NMR,ESI-MS and other spectral methods. Result: Totally 15 compounds were identified as mellein (1),linoleic acid (2),2-(2-hydroxyethyl)phenol (3),(3R)-mellein methyl ether (4),(3R,4S)-4-hydroxymellein (5),2-hydroxybenzaldehyde (6),3-phenylpropane-1,2-diol (7),1-phenyl-1,2-ethanediol (8),cyclo-(R-Prommmm-S-Ile) (9),cyclo-(D-Pro-L-Leu) (10),2-hydroxybenzaldehyde (11),4-hydroxy-8-O-methylmellein (12),cyclo-(S-Pro-S-Phe) (13),cyclo-(D-Pro-L-Ile) (14),cyclo-(D-Pro-L-Leu) (15). Conclusion: Compounds 1-15 were isolated from this fungus for the first time.
Résumé
Objective: To explore the efficacy and safety of chimeric antigen receptor T (CAR-T) cells in the treatment of central nervous system leukemia (CNSL). Methods: Two leukemia patients with CNSL were treated with CD19-CAR-T cells. The process and results of the entire treatment is reported and related literature review is conducted. Results: The patients were diagnosed as acute myeloid leukemia (AML)-M(2) with B lymphoid antigen expression and B cell acute lymphoblastic leukemia(B-ALL) by morphology and immunophenotype assay. The immunophenotype was consistent with the abnormal manifestations of AML-M(2) and B-ALL. Their clinical manifestations and laboratory tests met the diagnostic criteria of CNSL. The diagnosis was clear and the two patients were treated with CD19-CAR-T cell immunotherapy. Central nervous system symptoms were relieved. The imaging abnormalities of patient one has disappeared but cytokines release syndrome (CRS) occurred during the treatment. Cerebrospinal fluid of patient two was negative and no obvious CRS reaction was found. Conclusions: CAR-T cell immunotherapy is likely to induce the remission of CNSL and improve the prognosis.