Résumé
OBJECTIVE To study the causes of hospital acquired infections in the general hospitals and provide the reliable evidence to prevent and control the hospital acquired infections.METHODS By means of retrospective investigation,we observed 78829 patients with hospital acquired infections collected from May 2004 to Apr 2006 in our hospital.RESULTS The hospital acquired infection rate was 3.07%,the most cases were with respiratory infection,accounted for 59.07%,mainly with G-bacteria accounted for 49.73% and caused by primary malignancy,accounted for 18.05%.CONCLUSIONS The high risk factors of hospital acquired infections are immunosuppression,more invasive examinations and treatments and long-term using antibiotics.
Résumé
The coding region of S genome segment of Hantaan virus (76/118 strain) was inserted into the eukarytic expression plasmidpVR1012. The recombinant expression plasmid pVRS22 was constructed. Vero-E6 cells were transiently transfected in vitro with pVRS22 plasmid. The transient expression of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indirect immunofluorescence assay (IFA) with monoclonal antibody 5H5 against Hantaan virus.