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1.
Chinese Journal of Experimental and Clinical Virology ; (6): 445-447, 2011.
Article Dans Chinois | WPRIM | ID: wpr-246217

Résumé

<p><b>OBJECTIVE</b>To observe the HBV serum markers and HBV DNA expressions of the neonates born to the HBsAg-positive mothers.</p><p><b>METHODS</b>By detecting serum immunity markers of hepatitis B virus (5 items) and serum HBV DNA of 283 neonates (a pair of twins) born to 282 HBsAg-positive mothers.</p><p><b>RESULTS</b>12 patterns emerge from the study of the hepatitis B serum markers of 283 neonates. Topping the list is the combination of HBeAg and anti-HBc positive accounting for 48.41% (137/283), followed by the combination of anti-HBe and anti-HBc positive accounting for 22.26% (62/283). The third highest combination is that of HBsAg, HBeAg and anti-HBc positive accounting for 12.37% (35/283). There are five combinations accounting for 16.61% (47/283), each with HBsAg-positive. No case is found of the five items all negative or only HBsAb positive. Five cases are detected of serum HBV DNA > or = 1 x 103 IU/ml accounting for 1.77%.</p><p><b>CONCLUSIONS</b>Neonates born to HBsAg-positive mothers display complex patterns of serum hepatitis B markers, the dominant pattern being the combination of HBeAg and anti-HBc positive. Cases of serum HBV DNA > or = 1 x 10(3) IU/ml are rare.</p>


Sujets)
Femelle , Humains , Nouveau-né , Mâle , Marqueurs biologiques , Sang , ADN viral , Hépatite B , Anticorps de l'hépatite B , Sang , Antigènes de la nucléocapside du virus de l'hépatite virale B , Sang , Antigènes de surface du virus de l'hépatite B , Sang , Antigènes e du virus de l'hépatite virale B , Sang , Transmission verticale de maladie infectieuse
2.
Chinese Journal of Experimental and Clinical Virology ; (6): 461-463, 2010.
Article Dans Chinois | WPRIM | ID: wpr-231218

Résumé

<p><b>OBJECTIVE</b>To explore the clinical value of Lens culinaris agglutinin-reactive alpha-fetoprotein in the differentiation diagnosis between benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma.</p><p><b>METHODS</b>Alpha-fetoprotein variants from 300 patients with liver diseases were isolated with micro-spin column equipped lens culinaris agglutinin (LCA). The AFP and AFP-L3 were detected by the electrochemical luminescence (ECL) method, and the proportions of AFP-L3 were calculated.</p><p><b>RESULTS</b>The positive rates of AFP-L3 of HCC patients and chronic liver disease patients were 95% and 64% respectively, there were significant difference in two groups (chi2 = 134.72, P < 0.01), the HCC incidence rates of AFP-L3 positive and negative chronic liver disease patients showed significant difference (chi2 = 80.158, P < 0.01). there were no correlations between the proportion of AFP-L3 and AFP consistency(r = 0.046, P > 0.05).</p><p><b>CONCLUSIONS</b>The detection of AFP-L3 by micro-spin column assay show great clinical value in the differentiation diagnosis of benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma.</p>


Sujets)
Adolescent , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Enfant , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Marqueurs biologiques tumoraux , Carcinome hépatocellulaire , Diagnostic , Diagnostic différentiel , Maladies du foie , Diagnostic , Tumeurs du foie , Diagnostic , Lectines végétales , Chimie , Alphafoetoprotéines
3.
Chinese Journal of Experimental and Clinical Virology ; (6): 375-377, 2009.
Article Dans Chinois | WPRIM | ID: wpr-325537

Résumé

<p><b>OBJECTIVE</b>Explore the serum of patients with CHB of HBV large envelope protein (HBV-LHBs) trans-activation function and antiviral therapy effect relationship.</p><p><b>METHODS</b>60 cases of anti-viral treatment of patients with chronic hepatitis B to take every 3 months HBVDNA, HBV-LHBs, as well as detection of hepatitis B immune markers to observe the changes in indexes.</p><p><b>RESULTS</b>Income group 60 cases of anti-virus group HBVDNA with HBV-LHBs have a higher detection rate of the consistency of the results found no statistical significance (P > 0.05), HBV-LHBs-positive rate and positive rate of HBeAg differences (chi2 = 4.08, P < 0.05). After 24 months of antiviral therapy HBV-LHBs expression always HBVDNA in 29 cases of which occurred 24 months after the negative reaction of the 20 cases, continuous positive were seven cases of non-negative. 60 cases of patients 24 months found no HBsAg seroconversion, four cases of emergence of HBeAg seroconversion.</p><p><b>CONCLUSION</b>(1) detection of serum HBV-LHBs to reflect the hepatitis B virus replication with HBVDNA good correlation. (2) anti-viral treatment of dynamic observation of the process of HBV-LHBs expression can predict the effectiveness of anti-viral therapy.</p>


Sujets)
Adolescent , Adulte , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Antiviraux , Utilisations thérapeutiques , ADN viral , Sang , Génétique , Hépatite B , Sang , Traitement médicamenteux , Virologie , Antigènes de l'hépatite virale B , Sang , Génétique , Virus de l'hépatite B , Génétique , Physiologie , Résultat thérapeutique , Protéines de l'enveloppe virale , Sang , Génétique , Activation virale
4.
Chinese Journal of Experimental and Clinical Virology ; (6): 67-69, 2007.
Article Dans Chinois | WPRIM | ID: wpr-305492

Résumé

<p><b>OBJECTIVE</b>To evaluate the usefulness of new microspincolumn method for the measurement of a1pha-fetoprotein variant AFP-L3 in differentiation of benign and malignant liver disease and the warming for liver cancer.</p><p><b>METHODS</b>AFP-L3 was isolated by using microspincolumn coupled with lens culinaris agglutinin (LCA), AFP and AFP-L3 were determined with chemiluminescent immunoassay, the proportion of AFP-L3 levels AFP-L3(%) were calculated, and the relationship between the elevated AFP-L3(%) levels and benign and malignant liver disease was analyzed.</p><p><b>RESULTS</b>The levels of AFP-L3(%) in serum of patients with hepatocellular carcinoma was significantly higher than those in the patients with other liver diseases (P < 0.001). Taking AFP-L3(%) >or= 10% as the diagnostic criteria, the sensitivity for diagnosis of liver cancer was 90.9%.</p><p><b>CONCLUSION</b>Detection of AFP-L3 seemed to be of clinical value in diagnosis and differential diagnosis of hepatocellular carcinoma; it may be especially important for identifying patients with hepatocellular carcinoma whose a1pha-fetoprotein level is low.</p>


Sujets)
Adulte , Sujet âgé , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Carcinome hépatocellulaire , Sang , Diagnostic , Diagnostic différentiel , Hépatite chronique , Sang , Diagnostic , Dosage immunologique , Méthodes , Cirrhose du foie , Sang , Diagnostic , Tumeurs du foie , Sang , Diagnostic , Mesures de luminescence , Méthodes , Sensibilité et spécificité , Alphafoetoprotéines
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