Management of Airway Remodeling in a Mouse Model of Allergic Airways Inflammation Using Extracellular Vesicles from Human Bone Marrow-Derived Mesenchymal Stromal Cells

Abstract: Asthma is a chronic respiratory disease affecting 300 million people worldwide. It results in several structural changes in the airways, which are minimally accessible in clinical practice. Cell therapy using mesenchymal stromal cells (MSCs) is a promising strategy for treating asthma due to the paracrine activity of MSCs, which influences tissue regeneration and modulates the immune response. Studies using extracellular vesicles (EV) released by MSCs have demonstrated their regenerative properties in animal models. The aim of this study was to evaluate the potential of EVs isolated from human bone marrow MSCs (hBM-MSCs) to control lung tissue remodeling in ovalbumin-induced allergic asthma in Balb/c mice. We isolated hBM-MSCs from a single donor, expanded and characterized them, and then isolated EVs. Asthma was induced in 43 male Balb/c mice, divided into four groups: control, asthmatic (AS), asthmatic plus systemic EVs (EV-S), and asthmatic plus intratracheal EVs (EV-IT). Upon completion of asthma induction, animals were treated with EVs either locally (EV-IT) or intravenously (EV-S). Seven days after, we performed bronchoalveolar lavage (BAL) and the total nuclear cells were counted. The animals were euthanized, and the lungs were collected for histopathological analysis of the airways. The EV-S group showed improvement in only the total BAL cell count compared with the AS group, while the EV-IT group showed significant improvement in almost all evaluated criteria. Therefore, we demonstrate that the local application of EVs derived from hBM-MSCs may be a potential treatment in controlling asthma.

Espasmo de coronária por uso de cocaína / Espasm of coronary induced by cocaine use

Relato de um caso de espasmo de coronária induzido por cacaína, em paciente jovem com coronárias normais

Verapamil na crise hipertensiva / Verapamil in hypertensive crisis

Foram estudados 125 casos de crise hipertensiva, medicados com verapamil na dose de 5 a 10 mg por via venosa ou por infusäo gota a agota na dose de 25 mg durante 15 minutos. Todos os casos foram controlados através de monitorizaçäo da presäo arterial e freqüencia cardíaca e ECG nos tempos 0 (zero), 1,2,5,10,15,30,45 e60 minutos. Os resultados mostraram boa resposta anti-hipertensiva com iníco aos dois minutos e efeitos marcante entre 10 e 15 minutos, permanecendo até 60 minutos ou mais. Houve efeitos colaterais discretos de caráter transitório, sem repercussäo desfavorável