Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 1 de 1
Filtre
1.
The Korean Journal of Laboratory Medicine ; : 212-218, 2011.
Article Dans Anglais | WPRIM | ID: wpr-193614

Résumé

BACKGROUND: The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs). METHODS: The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors. HMSCs were cultured in DMEM with the addition of aPRP for 4 or 12 days; then, DNA content and ALP activity were measured. RESULTS: The quantity of DNA increased significantly at a 10% concentration of aPRP, but the ALP activity was suppressed at this concentration of aPRP. The GF concentrations varied among donors, and 5 subgroups of characteristic GF release patterns were identified via cluster analysis. DNA levels differed significantly between groups and tended to be higher in groups with higher concentrations of transforming growth factor-beta1 (TGF-beta1) and platelet-derived growth factors (PDGFs). DNA quantity was positively correlated with TGF-beta1 concentration, and was negatively correlated with donor age. ALP activity was negatively correlated with PDGF-BB concentration. CONCLUSIONS: The varying GF concentrations may result in different biologic effects; thus, individual differences in GF levels should be considered for reliable interpretation of the biologic functions and standardized application of PRP.


Sujets)
Humains , Phosphatase alcaline/métabolisme , Donneurs de sang , Différenciation cellulaire , Cellules cultivées , Milieux de culture/composition chimique , ADN/analyse , Protéines et peptides de signalisation intercellulaire/pharmacologie , Cellules souches mésenchymateuses/cytologie , Facteur de croissance dérivé des plaquettes/pharmacologie , Plasma riche en plaquettes/métabolisme , Facteur de croissance transformant bêta-1/pharmacologie
SÉLECTION CITATIONS
Détails de la recherche