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Acta cir. bras ; Acta cir. bras;30(2): 94-99, 02/2015. graf
Article de Anglais | LILACS | ID: lil-741029

RÉSUMÉ

PURPOSE: To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS: Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS: The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher. CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression. .


Sujet(s)
Animaux , Mâle , Brûlures/radiothérapie , Photothérapie de faible intensité/méthodes , Muscles squelettiques/effets des radiations , Protéine MyoD/analyse , Brûlures/métabolisme , Brûlures/anatomopathologie , Numération cellulaire , Modèles animaux de maladie humaine , Immunohistochimie , Fibres musculaires squelettiques/effets des radiations , Muscles squelettiques/anatomopathologie , Protéine MyoD/effets des radiations , Rat Wistar , Reproductibilité des résultats , Peau/traumatismes , Peau/effets des radiations , Facteurs temps , Résultat thérapeutique
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