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Journal of Forensic Medicine ; (6): 246-253, 2009.
Article Dans Chinois | WPRIM | ID: wpr-983479

Résumé

OBJECTIVE@#To observe the expression of Bcl-2 and Bax proteins in rat's myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning.@*METHODS@#Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system.@*RESULTS@#The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05).@*CONCLUSION@#If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.


Sujets)
Animaux , Femelle , Mâle , Rats , Modèles animaux de maladie humaine , Immunohistochimie , Myocarde/anatomopathologie , Myocytes cardiaques/anatomopathologie , Papaveraceae/composition chimique , Papavérine/intoxication , Protéines proto-oncogènes c-bcl-2/métabolisme , Rat Sprague-Dawley , Coloration et marquage , Facteurs temps , Protéine Bax/métabolisme
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