Résumé
<p><b>OBJECTIVE</b>To explore the expression of origin recognition complex 1 (ORC1) during the DNA replication of vascular muscle cells (VSMC).</p><p><b>METHODS</b>VSMC of thoracic aorta in rats were obtained by the adherence method of tissue culture. The cell synchrony was obtained by the method of double-thymidine block, colchicine treatment and serum starvation. The expression of ORC1 mRNA at different cell cycles of VSMC was determined by RT-PCR and the protein expression of ORC1 was analyzed by Western blot.</p><p><b>RESULTS</b>Cultured VSMC were identified by light microscope and immunocytochemistry. Significant expression of ORC1 mRNA and protein in a quiescent stage of VSMC were not observed. Upon synchronization, the expression of ORC1 mRNA was significantly higher at G(1)/S phase of VSMC than that at S and G(2)/M phases. The expression of ORC1 protein followed same changes as the ORC1 mRNA expression at different stages of cell cycles.</p><p><b>CONCLUSION</b>ORC1 may be an important regulatory factor at the initiation of proliferative process of VSMC.</p>
Sujets)
Animaux , Mâle , Rats , Aorte thoracique , Biologie cellulaire , Technique de Western , Cycle cellulaire , Prolifération cellulaire , Cellules cultivées , ADN , Génétique , Réplication de l'ADN , Régulation de l'expression des gènes tumoraux , Muscles lisses vasculaires , Biologie cellulaire , Myocytes du muscle lisse , Métabolisme , Complexe ORC , Génétique , Physiologie , ARN messager , Métabolisme , Rat Sprague-Dawley , RT-PCRRésumé
Studies on ORC in the cell cycle were limited,this paper is to explore origin recognition complex(ORC)in the cell cycle,and elucidate the relation between them.