A comparison of vessel sensitivity and vessel constant in oxygen uptake determination with pure oxygen and air as gaseous media.

Air proved to be a superior gaseous medium to pure oxygen used for oxygen uptake study in the Warburg apparatus Recording of volume change (vessel sensitivity method) was the only correct method of recording the oxygen uptake. It is concluded that the volume change method should be followed for recording oxygen uptake by Warburg apparatus and pure oxygen should not be used as a gaseous medium.

Relation between volume change and pressure change in oxygen uptake study using Warburg apparatus.

Taking oxygen and air as gaseous media oxygen uptake study was carried out recording delta V and corresponding delta P. From these data the quantity of oxygen consumed was calculated. It was concluded that delta V x vessel sensitivity x 2 = delta P x vessel constant. Recording of volume change in oxygen uptake study has been advocated.