Tumour necrosis factor alpha [TNF-Alpha] levels in toxoplasma gondii infected cases with or without repeated abortions

The aim of this study was to evaluate the tumor necrosis factor alpha [TNF-alpha] as one of cytokines produced in chronic toxoplasmosis in seropositive cases with anti-toxoplasma specific antibodies who have repeated abortions and in cases with normal delivery outcomes, compared to sero negative cases. TNF-alpha was found to be higher in seropositive cases with repeated abortion than in scronegative cases with repeated abortions and seronegative cases with normal delivery outcome

Serological diagnosis of trichinealla spiralis in experimentally infected mice

ELISA and IFA tests were used to detect IgG levels in sera of experimentally infected mice with Trichinella spiralis during a period of 12 weeks post infection. A crude saline extract of muscle larvae was used for application of ELISA technique, while the intact T. spiralis muscle larvae were used as an antigen for IFAT. In both tests, T spiralis IgG antibodies could be detected one week post infection in sera of all infected mice. ELISA test showed a sensitivity of 100% during the whole period of the experiment, while its specificity was 93.3%. Meanwhile, IFA test revealed a sensitivity of 100% and a specificity of 85%