Molecular characterization of Egyptian trichomonas vaginalis clinical isolates by HSP70 restriction fragment length polymorphism

Molecular typing of 20 Egyptian Trichomonas vaginalis clinical isolates was performed using the Restriction Fragment Length Polymorphism [RFLP] analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA. In each of the isolates tested 5 to 6 distinct DNA fragments ranging from 2.7 Kb to 7.5 Kb in size were detected. Analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct RFLP pattern subtypes of T. vaginalis. Eleven isolates [55%] showed the same RFLP pattern, teen of them [90.9%] were from symptomatic patients. T. vaginalis virus [TVV] was present in 7 isolates [35%]. Only one isolate was considered resistant to Metronidazole. There were no relations between TVV infection or Metronidazole susceptibility and RFLP subtypes

Biological variability of trichomonas vaginalis clinical isolates fom symptomatic and asymptomatic patients

The vaginal specimens of 60 women were cultured using modified thioglycolate medium. Thirteen samples were positive for Trichomonas vaginalis. The pathogenicity of these clinical isolates was investigated by intraperitoneal injection to mice. Additionally, their susceptibility to metronidazole was evaluated. All the clinical isolates produced infection in mice, but marked variations in pathogenicity and susceptibility were observed. Four out of 13 isolates produced mortality in mice, indicating a higher pathogenicity of the four isolates. Concerning metronidazole susceptibility, only one isolate was considered resistant and the other twelve showed different degrees of susceptibility

Femoral approach vs subclavian in measurement of central venous pressure in adult patients

Thirty patients with stable cardiovascular condition were included in this study. All the patients had already central venous catheter [either subclavian or internal jugular] in situ for measuring the SVCP. For all the patients, another catheter of the same type [multiple-lumen, 20 cm long] was inserted into the common iliac vein via the femoral route. The SVCP and CIVP were simultaneously measured at six-hour interval for 24 hours. It was concluded that CIVP measured by a 20 cm catheter placed through the femoral route can predict accurately the central venous pressure in adult patients [with normal intra-abdominal pressure] and it can be taken as a reliable alternative to SVCP and major complications