Effect of elicitors on the enhancement of podophyllotoxin biosynthesis in suspension cultures of linum album

The possibility of enhancment accumulation of podophyllotoxin [PTOX] has been studied in cultures of cell suspension of Linum album Kotschy ex Boiss. [Linaceae] producing PTOX was studied. Attempts were made to manipulate the biosynthetic pathway of PTOX by some biotic [yeast extract] and abiotic [Ag[+], Pb[2+] and Cd[2+]] elicitors for 24 or 48 hr in Murashige and Skoog [MS] medium. Silver significantly enhanced PTOX production up to 0.24% [mg/g cell dry wt.] in cultures. This effect could be attributed to the inhibitory role of silver on production of ethylene

Main phenolic compound of petals of Echium Amoenum fisch. and C. A. mey., a famous medicinal plant of Iran

Echium amoenum Fisch. and C.A. Mey. [Boraginaceae] is an indigenous Iranian plant, that its dry violet-blue petals [Gol-e-Gavzaban] have long been used in traditional medicine of Iran. In this study concentrated metanolic extract of the grounded dried petals of E. amoenum was fractionated by column chromatography and the fractions were purified by preparative HPLC. The structure of main pure component which was characterized by UV, IR, one and two dimensional 1H and 13C-NMR and Mass spectroscopy was found to be rosmarinic acid which is widespread in the plants of the Lamiaceae and Boraginaceae families in insignificant quantities and has antimicrobial, antiviral, and anti-inflammatory effects

Volatile constituents of a medicinal plant of Iran, echium amoenum Fisch. and C. A. Mey.

Echium amoenum Fisch. and C.A. Mey. [Boraginaceae] is an endemic Iranian plant, that its dry violet-blue petals has long been used in traditional medicine of Iran. The chemical composition of the volatile fraction of the dried petals of this plant which was isolated by steam distillation extraction with pentane [in yield of 0.05%] was examined by GC-MS. The constituents were identified by their mass spectra and Kovats' indices. The major components except aliphatic alkanes which belong to sesquiterpenes were: sigma -cadinene [24.25%], viridiflorol [4.9%], alpha -muurolene [4.52%], ledene [3.8%], alpha -calacorene [3.04%], and