Résumé
Experimental infection of laboratory animals with two "fixed" rabies virus strains by different routes. By feeding guimea pig brains infected with the Standard Challenge Virus (CVS) rabies strain, 2 albino rats and 1 white mouse became Infected. Transmission was not effected by feeding CVS infected guinea pig tissues to either rats or mice. Feeding of CVS infected mouse brains did not produce infection in rats and mice. The mean mouse LD50 dose by intramuscular injection was 10 2.25 while the corresponding mean mouse l/C LD50 ,dose qf theCVS mouse brain suspensions used was 10 8.07 . Transmission of the Pasteur Variant (PV) rabies virus by gastric intubation of concentrated infective sheep brain suspensions in rats and mice was successful. In addition, mice could also be infected by exposure to aerosols of the same infective materials. the mean mouse intramuscular LD 50 dose of the brain suspensions was 18.5per cent. The mean mouse intracerebral LD5o of the infected materials used was 10-5.1. Virus was not transmitted to untreated white mice kept in contact with infected ones. Confirmation of rabies infection was done by either the WHO Mouse inoculation test and or the fluorescent Antibody Test (F.A.T).
Sujets)
Virus de la rage , LaboratoiresRésumé
The first batch of lyophilized, Sample-type,beta propiolactone inactivated anti-rabies vaccine produced by B,P .I. in 1971 was used to immuaize 55 B.P .I. workers previously unexposed to rabies and with no history of rabies vaccination. Three doses of 0.25 ml of the vaccine were given intradermally at one week intervals. Booster doses given on the 98th.' 392nd. and 592nd. Day after the first dose. Blood samples were taken and serum-virus neutralization (SN) tests were performed at varying time intervals after basic immunization and booster doses. Satisfactory antibody responses were obtained. The course of immunological response is presented in tabular and graphical form. This work was carried out from June 1971 to January 1973.