Résumé
During 2000-2001 outbreaks of bovine ephemeral ever BEF occurred at Middle Egypt, 251 hepa-I rmzicd blood samples from cattle and buffaloes ah BEF symptoms were collected for virus iso-1011 and identification and 549 serum samples re collected for serological examination. Suck-g baby mice were intracerbrally [I/C] inocula-for virus isolation and identification, in the me lime isolated virus was adapted to grow in Vero and BKH[2] cell line. Identification isolated virus was achieved using both refer-lee and prepared hyper immune serum against:F virus in rabbit by using SPA agglutination est [SPA], Serum neutralization test [SNT], Mouse protection test [ MPT] and Agar gel precipitation test [AGFT]
Résumé
Inactivated tissue culture BEF virus vaccine was prepared from the 8th passage from a locally isolated BEF virus from native breed cattle during BEF outbreak in 2000 in Middle Egypt. The virus was inactivated using Binary ethylene amine [BEI] at concentration of 1% for 3.5 hours. The adjuvant contained mixture of Negilla Sativa oil, mineral oil and vitamin E and selenium with different ratio in tow different formula [A-vaccine ind B-vaccine]. The prepared vaccines were char-icterized using different techniques including, the irop test, emulsion stability and viscosity. In ad-fition its provide to:be sterile and safe. These vac-aes were compared with aluminum hydroxide fl inactive BEF virus vaccine. B-vaccine induce mime response in vaccinated cattle more than vaccine and aluminum hydroxide gel BEF vac-i, while control non-vaccinated groups did not pop any immune response against BEF virus when measure by SPA, VNT, and ELISA. Exposure of vaccinated cattle to natural infection with BEF virus revealed no clinical signs of BEF syndrome, while control non-vaccineted cattle groups developed clinical manifestation of BEF syndrome