A New Chromogenic Spray Reagent for Detection and Identification of Indoxacarb in Visceral Material by HPTLC

There is inherent rise of pesticide poisoning in India. Among all these pesticide indoxacarb is registered for use to control lepidopteran insects on crops, turf grasses, and landscape ornamentals. A novel chromatographic reagent is described for detection and identifications of Indoxacarb by HPTLC by use of furfural reagent. Indoxacarb (I) on acid hydrolysis yield its metabolites namely 4-trifloro-methoxy-phenyl amine (II), Oxidiazine (III) and acetic acid (IV). The 4-trifloro-methoxy-phenyl amine (II) under acidic condition reacts with furfural which gives grayish black colored spot on heating. The constituents of viscera (amino, acids, peptides, proteins, etc.) and plant materials do not interfere with the test. The detection limit for Indoxacarb is 0.5?g. The reaction mechanism is discussed. The necessary HPTLC parameter like concentration of analyte also mentioned for the efficient detection of indoxacarb pesticide.

Influenza surveillance in Pune, India, 2003.

Influenza surveillance was conducted in Pune, India in 2003. A total of 573 throat swabs/ nasal swabs (TS/NS) and 190 nasopharyngeal aspirates (NPA) were collected from 763 in- and out-patients who were mostly children aged 0-16 years. TS/NS (507/573) and NPA (42/190) specimens were processed in MDCK cell cultures and identified with the hemagglutination inhibition test (HI). A total of 37 influenza viruses was isolated: twenty-three type A (H3N2) and 14 type B of the Yamagata lineage were isolated from 29 children and 8 adults. Three type A (H3N2) isolates were characterized as being similar to A/Panama/2007/99 like, A/Korea/770/2000 like, and B/Sichuan/379/99 like strains.