First case of Dengue virus infection in Nepal.

Dengue virus transmitted by Aedes mosquito is one of the important cause health problems in world. Dengue fever and more severe and often fatal forms namely dengue hemorrhagic fever and dengue shock syndrome are emerging health problems in many part of the globe. No cases of Dengue virus infection have been reported from Nepal till date and for the first time, we report a case of dengue fever from Nepal.

Amplificación por PCR de una secuencia genomica de 272-pb que permite la diferenciación entre Trypanosoma cruzi y Trypanosoma rangeli / Annual reports 1994 / PCR amplification of a 272-bp genome sequence differentiates between Trypanosoma cruzi and Trypanosoma rangeli

La reacción en cadena de la polimerasa (PCR) fue empleada para amplificar un fragmento de 272-pb presente en el genoma del Trypanosoma cruzi, pero no detectado en el genoma del Trypanosoma rangeli. Para la amplificación por PCR se emplearon los oligonucleótidos TC4-1 y TC4-2 de 25-pb cada uno. Con el fin de aumentar la sensibilidad de la detección de los productos de amplificación en muestras biológicas, se preparó una sonda clonada en el vector pCR II y luego secuenciando el fragmento de 272-pb obtenido por amplificación del ADN de la cepa Tulahuen. Hemos confirmado por hibridación con la técnica Southern blot la no amplificación de ADN de T. rangeli con los cebadores TC4-1 y TC4-2. Estos resultados sugieren que esta técnica podría ser empleada para diferenciar infecciones por T. rangeli y T. cruzi

Detección basada en PCR de ADN de Trypanosoma Cruzi en suero / Annual Reports 1992 / PCR-Based detection of Trypanosoma Cruzi DNA in surem

DNA prepared from chagasic patientsïsera were amplified by the Polymerase Chain Reaction (PCR) using oligonucleotide primers which anneal specifically to a highly repetitive sequence of Trypanosoma Cruzi nuclear DNA. Sample from both acutely and chronically infected patients yielded positive results by this method. No significant difference was observed when either whole blood or serum sample of the patients were used. These results indicate that serum instead of whole blood sample could be used for PCR-based detection of T. Cruzi in field studies without the of applying any special chemical treatment of the specimens. This would represent a considerable advantage due to the easier handling and transportation of serum as compared with whole blood samples, especially in tropical climates

Clonación y secuenciación de AND de Trypanosoma cruzi derivado de PCR / Annual Reports 1991 / Cloning and sequencing of PCR-derived Trypanosoma cruzi DNA

A 188-bp DNA fragment obtained by PCR amplification using an oligonucleotide primer pair which anneal to the ends of a highly repetitive 155-bp Trypanosoma cruzi nuclear DNA was cloned in pUC18 and its sequences determined

Detencción por PCR del ADN de Trypanosoma cruzi con los pares de primers TC-4 y TC-22 / Annual Reports 1991 / Detection of Trypanosoma cruzi DNA with TC-4 and TC-22 primer pairs by PCR

chromosomal DNA from a Paraguayan Trypanosoma cruzi isolate was extracted and sonicated. The fragments were cloned and some clones were size-selectd and sequenced. Two primer pairs TC22 and TC4 were synthesized and applied to other Paraguayan T. cruzi isolates and two Leishmania mexicana amazoniensis strains by the polymerase chain reaction. The specificity and sensitivity of these primers were correlated with TCZ primer pair synthesized by Moser et al. Remarkable differences were observed between the three primer pair which could be possibly explained by the fact that TCZ primers unlike TC22 and TC4 amplify a highly repettitive sequence of chromosomal T. cruzi DNA

Seguimiento por PCR del tratamiento de un paciente chagástico agudo / Annual Reports 1991 / Follow-up of treatment of an acute chagasic patient by PCR

The polymerase chain reaction (PCR) was usedto evaluate the course of treatment of an acutely infected patient with Trypanosoma cruzi. The reactions were performed using a primer pair (TCZ1 and TCZ2) which prime the amplification of a highly repetive 195-bp T. cruzi genomic DNA. Treatment with benzonidazole (5 mg/kg boby weight) was strarted when parsitemia was 140 parsites per ml of blood. The strong DNA amplification bands initially observed were no longer detected after 8 days following treatment