Résumé
Chemical investigation of the methanol extract of Koordersiodendron pinnatum Merr. leaves resulted a new naphthalene derivative, (Z)-4-(tetradec-3-enyl)naphthalene-1,2,7-triol (1), together with three known compounds, β-sitosterol (2), 20-epibryonolic acid (3), and scopoletin (4). The structure of the new compound was elucidated based on spectroscopic evidence. The isolated compounds (1-4) were tested their cytotoxic activities against the P-388 murine leukemia cell line and compound 1 has highest activity with IC50 1.94 ㎛
Résumé
Chemical investigation of the methanol extract of Koordersiodendron pinnatum Merr. leaves resulted a new naphthalene derivative, (Z)-4-(tetradec-3-enyl)naphthalene-1,2,7-triol (1), together with three known compounds, β-sitosterol (2), 20-epibryonolic acid (3), and scopoletin (4). The structure of the new compound was elucidated based on spectroscopic evidence. The isolated compounds (1-4) were tested their cytotoxic activities against the P-388 murine leukemia cell line and compound 1 has highest activity with IC50 1.94 ㎛
Résumé
Aims: Diarrheal disease is one of serious healthcare problems in developing countries. Endophytic bacteria have been known as a promising source of new antibiotics against susceptible and resistant forms of microorganisms. In this study, we identified three endophytic bacteria isolated from Neesia altissima and screened their antagonistic activity against diarrhea-causing bacteria in order to find new potential secondary metabolites. Methodology and results: Samples of N. altissima were collected from mount Halimun-Salak national park. Endophytic bacteria were isolated from roots, barks, and fresh leaves of N. altissima by surface sterilized method. Screening of antagonistics activity was conducted against five diarrhea-causing bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Shigella flexneri, and Bacillus cereus using crude extract dilution and diffusion disc methods. Three endophytic bacteria showed antagonistic activity against the pathogenic bacteria. Identification of the three potential endophytic bacteria using molecular analysis showed that two isolates determined as Pseudomonas aeruginosa and one isolate belongs to P. azotoformans. Conclusion, significance and impact study: Crude extract of P. aeruginosa (strain 1.4.1A and 1.2.7D) and P. azotoformans (strain 1.8.7KB) showed growth inhibition activity to the diarrhea-causing bacteria. This is the first report of P. azotoformans exhibited antagonistic activities against diarrhea-causing bacteria. This data suggested that bacterial endophytes from N. altissima provided potential sources for the discovery of new secondary metabolites to combat the diarrhea-causing bacteria. This finding highlights potential prospects of endophytic bacteria utilization from endemic medicinal plants for the discovery of novel bioactive compounds.
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BactériesRésumé
HDL-cholesterol raising effect of atorvastatin was compared to that of dehydrolovastatin in an 8-week study in 5 equally-membered groups of pre-acclimatized Sprague-Dawley rats. The first and second groups were normal and hyperlipidemic control, while the others were treated with atorvastatin 14.4 mg, dehydrolovastatin 7.2 mg, and dehydrolovastatin 14.4 mg, per 200 g of mouse body weight per day, respectively. Slightly better effect than atorvastatin\ s could only be achieved by dehydrolovastatin with the same dose.