Sublethal toxicity of carbofuran in cattle egret (Bubulcus ibis coromandus): hematological, biochemical, and histopathological alterations / Toxicidade subletal do carbofurano na garça-vaqueira (Bubulcus ibis coromandus): alterações hematológicas, bioquímicas e histopatológicas

Abstract This study was aimed to investigate Carbofuran (CF)-induced pathological changes in cattle egret. Two hundred cattle egrets were reared and equally divided into four groups and given different CF concentrations (0.03 mg/L, 0.02 mg/L, 0.01 mg/L and 0 mg/L (control group)). Hematology, serum biochemistry, histopathology, and immunological markers were studied. Our results confirm that CF induces anemic conditions, leukocytosis, elevated liver enzymatic activity, and alterations in renal biomarkers. Moreover, specific microscopic lesions such as multifocal necrosis, pyknotic nuclei, hemorrhages, congestion, and inflammatory cell proliferation were observed in the liver, kidney, spleen, and thymus. These findings suggest that CF can induce harmful effects, so the application of this pesticide in the field must be strictly monitored to mitigate the possibility of exposure to non-target species.

Resumo Este estudo teve como objetivo investigar as alterações patológicas induzidas por carbofurano (CF) em garças-vaqueiras. Duzentas dessas garças foram criadas e divididas igualmente em quatro grupos e receberam diferentes concentrações de CF: 0,03 mg/L; 0,02 mg/L; 0,01 mg/L; e 0 mg/L (grupo controle). Foram realizadas análises de hematologia, bioquímica sérica, histopatologia e marcadores imunológicos. Nossos resultados confirmaram que CF induz condições anêmicas, leucocitose, atividade enzimática hepática elevada e alterações nos biomarcadores renais. Além disso, lesões microscópicas específicas, como necrose multifocal, núcleos picnóticos, hemorragias, congestão e proliferação de células inflamatórias, foram observadas no fígado, rim, baço e timo. Esses achados sugerem que o CF pode causar efeitos nocivos, portanto a aplicação desse agrotóxico no campo deve ser rigorosamente monitorada para mitigar a possibilidade de exposição a espécies não alvo.

Sublethal toxicity of carbofuran in cattle egret (Bubulcus ibis coromandus): hematological, biochemical, and histopathological alterations

Abstract This study was aimed to investigate Carbofuran (CF)-induced pathological changes in cattle egret. Two hundred cattle egrets were reared and equally divided into four groups and given different CF concentrations (0.03 mg/L, 0.02 mg/L, 0.01 mg/L and 0 mg/L (control group)). Hematology, serum biochemistry, histopathology, and immunological markers were studied. Our results confirm that CF induces anemic conditions, leukocytosis, elevated liver enzymatic activity, and alterations in renal biomarkers. Moreover, specific microscopic lesions such as multifocal necrosis, pyknotic nuclei, hemorrhages, congestion, and inflammatory cell proliferation were observed in the liver, kidney, spleen, and thymus. These findings suggest that CF can induce harmful effects, so the application of this pesticide in the field must be strictly monitored to mitigate the possibility of exposure to non-target species.

Resumo Este estudo teve como objetivo investigar as alterações patológicas induzidas por carbofurano (CF) em garças-vaqueiras. Duzentas dessas garças foram criadas e divididas igualmente em quatro grupos e receberam diferentes concentrações de CF: 0,03 mg/L; 0,02 mg/L; 0,01 mg/L; e 0 mg/L (grupo controle). Foram realizadas análises de hematologia, bioquímica sérica, histopatologia e marcadores imunológicos. Nossos resultados confirmaram que CF induz condições anêmicas, leucocitose, atividade enzimática hepática elevada e alterações nos biomarcadores renais. Além disso, lesões microscópicas específicas, como necrose multifocal, núcleos picnóticos, hemorragias, congestão e proliferação de células inflamatórias, foram observadas no fígado, rim, baço e timo. Esses achados sugerem que o CF pode causar efeitos nocivos, portanto a aplicação desse agrotóxico no campo deve ser rigorosamente monitorada para mitigar a possibilidade de exposição a espécies não alvo.

Impact of diet on attention-deficit hyperactivity disorder in a tertiary care hospital of Rawalpindi

Background: Attention-deficit hyperactivity disorder (ADHD) is a prevalent neuropsychiatric disorder of children that affects almost all aspects of the life. The role of diet in the management of the ADHD is understudied in Pakistan. This study aimed to assess the impact of different types of diet on the symptoms of ADHD among the children with ADHD. Methods: This descriptive cross-sectional study was conducted among 58 children with ADHD in Rawalpindi. Ethical approval and informed consent were obtained. Patients’ recruitment was done via developed criteria and Convenient sampling technique. Descriptive statistics and one-way ANOVA were used to determine the difference of ADHD severity among various frequencies of different included foods via Conner Parent rating scale-revised short form. Data analysis was performed by SPSS version 25.0. Results: The difference in hyperactivity index, impulsivity, and learning problems score on CPR-RS among various frequencies of carbohydrate, protein rich and fat foods, was statistically significant and with the increase intake of carbohydrate and protein rich foods, the score on CPR-RS of hyperactivity index, impulsivity, and learning problems also goes up. Whereas, with the increase intake of fat rich foods, the score of hyperactivity index, impulsivity, and learning problems goes down. Conclusions: In short, the high intake of carbohydrate and protein rich foods, increase the severity of the symptoms of ADHD, while, high intake of fat rich foods decreases the severity of the symptoms of ADHD among children.

Impact of breastfeeding on the intelligence quotient of the children of Rawalpindi, Pakistan

Background: Breastfeeding impacts the children physical and mental growth and development. The impact of breastfeeding on intelligence quotient (IQ) of children is under-researched in Pakistan. This study aimed to determine the impact of breastfeeding on the intelligence quotient among children of Rawalpindi, Pakistan. Methods: This comparative cross-sectional was performed among 88 children of the Rawalpindi. Patients’ enrolment in the study was done via non-probability convenient sampling and developed inclusion and exclusion criteria. Ethical approval and informed consent were taken. Intelligence quotient was calculated through Wechsler intelligence scale for children (WISC). Data analysis was done via descriptive and inferential statistics by statistical package for the social sciences (SPSS) version 25. Chi square test was used to assess the variables association. P value less than 0.05 was set as statistically significant. Results: The overall mean score on WISC of intelligence quotient was 106 with SD of ±39, whereas, mean score of intelligence quotient for breastfed children was 112 with SD of ±32 and mean score of intelligence for children who were on formula milk was 100 with SD of ±36. The association between the type of milk and intelligence quotient (p=0.003) and association between duration of breastfeeding and intelligence quotient (p=0.04) was statistically significant. Conclusions: Children who had been on breastfeeding had better IQ. Furthermore, children who were breastfed for longer duration had even better IQ than children who were on breastfeeding for shorter duration.